Dr. Piotr  Walczak

Dr. Piotr Walczak

Associate Professor
Kennedy Krieger Institute, USA


Highest Degree
Ph.D. in Medicine from University of Warmia and Mazury, Poland

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Area of Interest:

Medicine
100%
Neuroscience Research
62%
Magnetic Resonance in Medicine
90%
Stem Cells
75%
Molecular Imaging
55%

Selected Publications

  1. Xu, J., N.N. Yadav, A. Bar-Shir, C.K. Jones and K.W. Chan et al., 2013. Variable delay multi-pulse train for fast chemical exchange saturation transfer and relayed-nuclear overhauser enhancement MRI. Magn. Reson. Med.,. 10.1002/mrm.24850.
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  2. Nowakowski, A., A. Andrzejewska, M. Janowski, P. Walczak and B. Lukomska, 2013. Genetic engineering of stem cells for enhanced therapy. Acta Neurobiol. Exp. (Wars), 73: 1-18.
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  3. Liang, Y., P. Walczak and J.W. Bulte, 2013. The survival of engrafted neural stem cells within hyaluronic acid hydrogels. Biomaterials, 34: 5521-5529.
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  4. Liang, Y., L. Agren, A. Lyczek, P. Walczak and J.W. Bulte, 2013. Neural progenitor cell survival in mouse brain can be improved by co-transplantation of helper cells expressing bFGF under doxycycline control. Exp. Neurol., 247C: 73-79.
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  5. Janowski, M., C. Engels, M. Gorelik, A. Lyczek, S. Bernard, J.W. Bulte and P. Walczak, 2013. Survival of neural progenitors allografted into the CNS of immunocompetent recipients is highly dependent on transplantation site. Cell Trans.,. .
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  6. Janowski, M., A. Lyczek, C. Engels, J. Xu, B. Lukomska, J.W. Bulte and P. Walczak, 2013. Cell size and velocity of injection are major determinants of the safety of intracarotid stem cell transplantation. J. Cereb. Blood Flow Metab., 33: 921-927.
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  7. Chan, K.W., G. Liu, X. Song, H. Kim and T. Yu et al., 2013. MRI-detectable pH nanosensors incorporated into hydrogels for in vivo sensing of transplanted-cell viability. Nat. Mater., 12: 268-275.
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  8. Barczewska, M., J. Wojtkiewicz, A. Habich, M. Janowski and Z. Adamiak et al., 2013. MR monitoring of minimally invasive delivery of mesenchymal stem cells into the porcine intervertebral disc. PLoS One, Vol. 8. 10.1371/journal.pone.0074658.
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  9. Bar-Shir, A., G. Liu, Y. Liang, N.N. Yadav and M.T. McMahon et al., 2013. Transforming thymidine into a magnetic resonance imaging probe for monitoring gene expression. J. Am. Chem. Soc., 135: 1617-1624.
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  10. Link, T.W., D. Arifin, C.M. Long, P. Walczak, N. Muja, A. Arepally and J.W. Bulte, 2012. Use of magnetocapsules for in vivo visualization and enhanced survival of xenogeneic HepG2 cell transplants Cell. Medicine, 4: 77-84.
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  11. Liang, Y., P. Walczak and J.W. Bulte, 2012. Comparison of red-shifted firefly luciferase Ppy RE9 and conventional Luc2 as bioluminescence imaging reporter genes for in vivo imaging of stem cells. J. Biomed. Opt., Vol. 17. 10.1117/1.JBO.17.1.016004.
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  12. Kim, H., P. Walczak, N. Muja, J.T. Campanelli and J.W. Bulte, 2012. ICV-transplanted human glial precursor cells are short-lived yet exert immunomodulatory effects in mice with EAE. Glia, 60: 1117-1129.
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  13. Kim, H., P. Walczak, C. Kerr, C. Galpoththawela, A.A. Gilad, N. Muja and J.W.M. Bulte, 2012. Immunomodulation by transplanted human embryonic stem cell-derived oligodendroglial progenitors in experimental autoimmune encephalomyelitis. Stem Cells, 30: 2820-2829.
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  14. Kedziorek, D.A., L.V. Hofmann, Y. Fu, W.D. Gilson and K.M. Cosby et al., 2012. X-ray-visible microcapsules containing mesenchymal stem cells improve hind limb perfusion in a rabbit model of peripheral arterial disease. Stem Cells, 30: 1286-1296.
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  15. Janowski, M., A. Jablonska, H. Kozlowska, I. Orukari and S. Bernard et al., 2012. Neonatal desensitization is not a universal method for preventing xenograft rejection. Nat. Meth., 9: 856-858.

  16. Gorelik, M., M. Janowski, C. Galpoththawela, R. Rifkin and M. Levy et al., 2012. Noninvasive monitoring of immunosuppressive drug efficacy to prevent rejection of intracerebral glial precursor allografts. Cell Trans., 21: 2149-2157.
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  17. Gorelik, M., I. Orukari, J. Wang, C. Galpoththawela and H. Kim et al., 2012. Use of MR cell tracking to evaluate targeting of glial precursor cells to inflammatory tissue by exploiting the very late antigen-4 docking receptor. Radiology, 265: 175-185.
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  18. Cromer Berman, S.M., Kshitiz, J. Wang, I. Orukari, A. Levchenko, J.W. Bulte and P. Walczak, 2012. Cell motility of neural stem cells is reduced after spio-labeling, which is mitigated after exocytosis. Magn. Reson. Med., 69: 255-262.
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  19. Aarntzen, E.H., M. Srinivas, P. Walczak, M. Janowski and A. Heerschap et al., 2012. In vivo tracking techniques for cellular regeneration, replacement and redirection. J. Nucl. Med., 53: 1825-1828.
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  20. Walczak, P., A.H. All, N. Rumpal, M. Gorelik and H. Kim et al., 2011. Human glial-restricted progenitors survive, proliferate and preserve electrophysiological function in rats with focal inflammatory spinal cord demyelination. Glia, 59: 499-510.
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  21. Muja, N., M.E. Cohen, J. Zhang, H. Kim and A.A. Gilad et al., 2011. Neural precursors exhibit distinctly different patterns of cell migration upon transplantation during either the acute or chronic phase of EAE: A serial MR imaging study. Magn. Reson. Med., 65: 1738-1749.
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  22. Liu, G., Y. Liang, A. Bar-Shir, K.W. Chan and C.S. Galpoththawela et al., 2011. Monitoring enzyme activity using a diamagnetic chemical exchange saturation transfer magnetic resonance imaging contrast agent. J. Am. Chem. Soc., 133: 16326-16329.
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  23. Liu, G., M. Moake, Y.E. Har-El, C.M. Long and K.W. Chan et al., 2011. In vivo multicolor molecular MR imaging using diamagnetic chemical exchange saturation transfer liposomes. Magn. Res. Med., 67: 1106-1113.
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  24. Li, N., J.E. Downey, A. Bar-Shir, A.A. Gilad and P. Walczak et al., 2011. Optogenetic-guided cortical plasticity after nerve injury. PNAS, 108: 8838-8843.
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  25. Berman, S.M., P. Walczak and J.W. Bulte, 2011. MRI of transplanted neural stem cells. Meth. Mol. Biol., 711: 435-449.
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  26. Berman, S.C., C. Galpoththawela, A.A. Gilad, J.W. Bulte and P. Walczak, 2011. Long-term MR cell tracking of neural stem cells grafted in immunocompetent versus immunodeficient mice reveals distinct differences in contrast between live and dead cells. Magn. Res. Med., 65: 564-574.
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  27. Barnett, B.P., J. Ruiz-Cabello, P. Hota, R. Ouwerkerk and M.J. Shamblott et al., 2011. Use of perfluorocarbon nanoparticles for non-invasive multimodal cell tracking of human pancreatic islets. Contrast Med. Mol. Imaging, 6: 251-259.
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  28. Barnett, B.P., J. Ruiz-Cabello, P. Hota, R. Liddell and P. Walczak et al., 2011. Fluorocapsules for improved function, immunoprotection and visualization of cellular therapeutics with MR, US and CT imaging. Radiology, 258: 182-191.
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  29. Xie, D., B. Qiu, P. Walczak, X. Li and J. Ruiz-Cabello et al., 2010. Optimization of magnetosonoporation for stem cell labeling. NMR Biomed., 23: 480-481.
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  30. Qiu, B., D. Xie, P. Walczak, X. Li, J. Ruiz-Cabello, S. Minoshima, J.W. Bulte and X. Yang, 2010. Magnetosonoporation: Instant magnetic labeling of stem cells. Magn. Reson. Med., 63: 1437-1441.
    CrossRef  |  

  31. Kedziorek, D.A., N. Muja, P. Walczak, J. Ruiz-Cabello, A.A. Gilad, C.C. Jie and J.W. Bulte, 2010. Gene expression profiling reveals early cellular responses to intracellular magnetic labeling with superparamagnetic iron oxide nanoparticles. Magn. Reson. Med., 63: 1031-1043.
    CrossRef  |  

  32. Janowski, M., P. Walczak and I. Date, 2010. Intravenous route of cell delivery for treatment of neurological disorders: A meta-analysis of preclinical results. Stem Cells Dev., 19: 5-16.
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  33. Engberink, R.D., S.M. van der Pol, P. Walczak, A. van der Toorn and M.A. Viergever et al., 2010. Magnetic resonance imaging of monocytes labeled with ultrasmall superparamagnetic particles of iron oxide using magnetoelectroporation in an animal model of multiple sclerosis. Mol. Imaging, 9: 268-277.
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  34. All, A.H., G. Agrawal, P. Walczak, A. Maybhate, J.W. Bulte and D.A. Kerr, 2010. Evoked potential and behavioral outcomes for experimental autoimmune encephalomyelitis in Lewis rats. Neurol. Sci., 31: 595-601.
    CrossRef  |  

  35. Gilad, A.A., H.W. van Laarhoven, M.T. McMahon, P. Walczak and A. Heerschap et al., 2009. Feasibility of concurrent dual contrast enhancement using CEST contrast agents and superparamagnetic iron oxide particles. Magn. Reson. Med., 61: 970-974.
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  36. All, A.H., P. Walczak, G. Agrawal, M. Gorelik and C. Lee et al., 2009. Effect of MOG sensitization on somatosensory evoked potential in Lewis rats. J. Neurol. Sci., 284: 81-89.
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  37. Walczak, P., J. Zhang, A.A. Gilad, D.A. Kedziorek and J. Ruiz-Cabello et al., 2008. Dual-modality monitoring of targeted intraarterial delivery of mesenchymal stem cells after transient ischemia. Stroke, 39: 1569-1574.
    CrossRef  |  Direct Link  |  

  38. Ruiz-Cabello, J., P. Walczak, D.A. Kedziorek, V.P. Chacko and A.H. Schmieder et al., 2008. In vivo hot spot MR imaging of neural stem cells using fluorinated nanoparticles. Magn. Reson. Med., 60: 1506-1511.
    CrossRef  |  

  39. Mikhaylova, M., N. Mori, F.B. Wildes, P. Walczak, B. Gimi and Z.M. Bhujwalla, 2008. Hypoxia increases breast cancer cell-induced lymphatic endothelial cell migration. Neoplasia, 10: 380-389.
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  40. Korosoglou, G., R.G. Weiss, D.A. Kedziorek, P. Walczak, W.D. Gilson et al., 2008. Noninvasive detection of macrophage-rich atherosclerotic plaque in hyperlipidemic rabbits using positive contrast magnetic resonance imaging. J. Am. Coll. Cardiol., 52: 483-491.
    CrossRef  |  

  41. Gilad, A.A., P. Walczak, M.T. McMahon, H.B. Na and J.H. Lee et al., 2008. MR tracking of transplanted cells with positive contrast using manganese oxide nanoparticles. Magn. Reson. Med., 60: 1-7.
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  42. Walczak, P., N. Chen, D. Eve, J. Hudson and T. Zigova et al., 2007. Long-term cultured human umbilical cord neural-like cells transplanted into the striatum of NOD SCID mice. Brain Res. Bull., 74: 155-163.
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  43. Walczak, P., D.A. Kedziorek, A.A. Gilad, B.P. Barnett and J.W. Bulte, 2007. Applicability and limitations of MR tracking of neural stem cells with asymmetric cell division and rapid turnover: The case of the Shiverer dysmyelinated mouse brain. Magn. Reson. Med., 58: 261-269.
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  44. Qiu, B., F. Gao, P. Walczak, J. Zhang, S. Kar, J.W. Bulte and X. Yang, 2007. In vivo MR imaging of bone marrow cells trafficking to atherosclerotic plaques. J. Magn. Reson. Imaging, 26: 339-348.
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  45. Gilad, A.A., M.T. McMahon, P. Walczak, P.T. Winnard and V. Raman Jr. et al., 2007. Artificial reporter gene providing MRI contrast based on proton exchange. Nat. Biotechnol., 25: 217-219.
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  46. Gao, F., S. Kar, J. Zhang, B. Qiu and P. Walczak et al., 2007. MRI of intravenously injected bone marrow cells homing to the site of injured arteries. NMR Biomed., 20: 673-681.
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  47. Barnett, B.P., A. Arepally, P.V. Karmarkar, D. Qian, W.D. Gilson et al., 2007. Magnetic resonance-guided, real-time targeted delivery and imaging of magnetocapsules immunoprotecting pancreatic islet cells. Nat. Med., 13: 986-991.
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  48. Walczak, P., J. Ruiz-Cabello, D.A. Kedziorek, A.A. Gilad and S. Lin et al., 2006. Magnetoelectroporation: Improved labeling of neural stem cells and leukocytes for cellular magnetic resonance imaging using a single FDA-approved agent. Nanomedicine, 2: 89-94.
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  49. Lepore, A.C., P. Walczak, M.S. Rao, I. Fischer and J.W. Bulte, 2006. MR imaging of lineage-restricted neural precursors following transplantation into the adult spinal cord. Exp. Neurol., 201: 49-59.
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  50. Barnett, B.P., D.L. Kraitchman, C. Lauzon, C.A. Magee and P. Walczak et al., 2006. Radiopaque alginate microcapsules for X-ray visualization and immunoprotection of cellular therapeutics. Mol. Pharm., 3: 531-538.
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  51. Walczak, P., D.A. Kedziorek, A.A. Gilad, S. Lin and J.W. Bulte, 2005. Instant MR labeling of stem cells using magnetoelectroporation. Magn. Reson. Med., 54: 769-774.
    CrossRef  |  

  52. Kraitchman, D.L., M. Tatsumi, W.D. Gilson, T. Ishimori and D. Kedziorek et al., 2005. Dynamic imaging of allogeneic mesenchymal stem cells trafficking to myocardial infarction. Circulation, 112: 1451-1461.
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  53. Chen, N., J.E. Hudson, P. Walczak, I. Misiuta and S. Garbuzova-Davis et al., 2005. Human umbilical cord blood progenitors: The potential of these hematopoietic cells to become neural. Stem Cells, 23: 1560-1570.
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  54. Walczak, P., N. Chen, J.E. Hudson, A.E. Willing and S.N. Garbuzova-Davis et al., 2004. Do hematopoietic cells exposed to a neurogenic environment mimic properties of endogenous neural precursors? J. Neurosci. Res., 76: 244-254.
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  55. Hudson, J.E., N. Chen, S. Song, P. Walczak and P. Jendelova et al., 2004. Green fluorescent protein bone marrow cells express hematopoietic and neural antigens in culture and migrate within the neonatal rat brain. J. Neurosci. Res., 76: 255-264.
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