Biography

I am currently serving as Professor of Biosciences at Islamabad Campus. I have served CIIT for more than 12 years now. In fact, I was the first faculty member at Biosciences and played a significant role in setting up and strengthening Department of Biosciences, in 2002-2003, here at COMSATS Institute of Information Technology, Islamabad. I am mainly responsible for starting of a teaching/research program in the then brand new field of Bioinformatics in Pakistan.
In the area of Microbial Genomics, I am the first researcher from Pakistan who has been able to complete the whole genome sequence of 100 Vibrio cholerae isolates (100 Genome Project) as well of Campylobacter jejuni from Pakistan in collaboration with Sanger centre, UK. The data generated will be used by the Bioinformatics students in the country for their training and skill development and can also play a pivotal role in strengthening software development industry in Pakistan. Furthermore, Diagnostic kit for the rapid detection of Vibrio strains hired responsible for disease will be designed based on the data generated. I have also identified couple of novel fluorescent proteins which has the commercial value for the scientific community.
Since my joining to CIIT, I have not only designed and introduced a brand new degree program of “Bioinformatics” but was also key person in setting up and strengthening Department of Biosciences with its state of the art laboratories. I have supervised and co-supervised numerous undergraduate (40 projects) and post graduate projects (25) and has published numerous papers (>60) and submitted about 10 papers till to date to international impact factor journals. I was mainly responsible for preparing first “National Bioinformatics policy” document in 2004 submitted to HEC. I have developed several important international links and has opened up opportunities for staff and students to work in Foreign Universities of highest ranking in the world. I have been a affiliate faculty and visiting scientist in the US and UK. During my research career at COMSATS since 2003, I have won number of national and international research fundings.

Area of Interest:

Molecular Sciences

100%

Virulence

62%

Mucosal Pathogens Transmission

90%

Host-Pathogen Interaction

75%

Vaccines Surveillance

55%

Research Publications in Numbers

Books
0

Chapters
0

Articles
0

Abstracts
0

Selected Publications

Jamil, B., H. Habib, S. Abbasi, H. Nasir and A. Rahman et al., 2016. Cefazolin loaded chitosan nanoparticles to cure multi drug resistant Gram-negative pathogens. Carbohydr. Polym., 136: 682-691.
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Tariq, A., A. Hameed, H. Bokhari and F. Masood, 2015. Is atomic rearrangement of type IV PHA synthases responsible for increased PHA production?. J. Biomol. Struct. Dyn., 33: 1225-1238.
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Siddiqui, F.M., M. Ibrahim, N. Noureen, Z. Noreen and R.W. Titball et al., 2015. Draft Genome Sequence of the Enteropathogenic Bacterium Campylobacter jejuni Strain cj255. Genome announcements, Vol. 3 10.1128/genomeA.01223-15.
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Siddiqui, F.M., M. Akram, N. Noureen, Z. Noreen and H. Bokhari, 2015. Antibiotic susceptibility profiling and virulence potential of Campylobacter jejuni isolates from different sources in Pakistan. Asian Pac. J. Trop. Med., 8: 197-202.
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Siddiqui, F., O. Champion, M. Akram, D. Studholme and S.A.M.A.S. Eqani et al., 2015. Molecular detection identified a type six secretion system in Campylobacter jejuni from various sources but not from human cases. J. Appl. Microbiol., 118: 1191-1198.
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Riaz, M.R., A.R. Siddiqi and H. Bokhari, 2015. Structural and functional studies of BapC protein of Bordetella pertussis. Microbiol. Res., 174: 56-61.
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Noreen, Z., M. Abrar, F. Siddiqui, R. Faryal, H. Hamid and H. Bokhari, 2015. Antibiotic susceptibility and molecular characterization of campylobacter jejuni strain isolated from a guillain barre syndrome child. Indian J. Pediatr., 10.1007/s12098-015-1923-z.
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Mirza, S.B., H. Bokhari and M.Q. Fatmi, 2015. Exploring Natural Products from the Biodiversity of Pakistan for Computational Drug Discovery Studies: Collection, Optimization, Design and Development of A Chemical Database (ChemDP). Curr. Comput., 11: 102-109.
Masood, F., T. Yasin, H. Bukhari and M. Mujahid, 2015. Characterization and application of roxithromycin loaded cyclodextrin based nanoparticles for treatment of multidrug resistant bacteria. Mater. Sci. Eng: C, 61: 1-7.
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Javed, S., F. Said, S. EQANI and H. Bokhari, 2015. Bordetella parapertussis outbreak in Bisham, Pakistan in 2009-2010: fallout of the 9/11 syndrome. Epidemiol. Infect., 143: 2619-2623.
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Jamil, B., H. Bokhari and M. Imran, 2015. Mechanism of Action: How Nano-antimicrobials Act?. Curr. Drug Targets, Vol. 16 10.2174/1389450116666151019101826.
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Bhowmik, A.K., A. Alamdar, I. Katsoyiannis, H. Shen and N. Ali et al., 2015. Mapping human health risks from exposure to trace metal contamination of drinking water sources in Pakistan. Sci. Total Environ., 538: 306-316.
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Abdullah, M., M. Fasola, A. Muhammad, S.A. Malik and N. Bostan et al., 2015. Avian feathers as a non-destructive bio-monitoring tool of trace metals signatures: A case study from severely contaminated areas. Chemosphere, 119: 553-561.
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Syed, J.H., A. Alamdar, A. Mohammad, K. Ahad and Z. Shabir et al., 2014. Pesticide residues in fruits and vegetables from Pakistan: A review of the occurrence and associated human health risks. Environ. Sci. Pollut. Res., 21: 13367-13393.
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Shah, M.A., A. Mutreja, N. Thomson, S. Baker and J. Parkhill et al., 2014. Genomic Epidemiology of Vibrio cholerae O1 Associated with Floods, Pakistan, 2010. Emerging Infect. Dis., 20: 13-20.
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Mumtaz, S., R. Hussain, A. Rauf, M.Q. Fatmi, H. Bokhari, M. Oelgemoller and A.M. Qureshi, 2014. Synthesis, molecular docking studies, and in vitro screening of barbiturates/thiobarbiturates as antibacterial and cholinesterase inhibitors. Med. Chem. Res., 23: 2715-2726.
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Khan, M., A. Mohammad, K. Ahad, A. Katsoyiannis and S.A. Malik et al., 2014. Cattle egrets as a biosentinels of persistent organic pollutants exposure. Environ. Geochem. Health, 36: 375-384.
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Idrees, M., K. Thangavelu, M. Sikaroodi, C. Smith, J. Sivaraman, P.M. Gillevet and H. Bokhari, 2014. Novel fluorescent protein from Hydnophora rigida possesses green emission. Biochem. Biophys. Res. Commun., 448: 33-33.
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Harrison, J.W., T.T.N. Dung, F. Siddiqui, S. Korbrisate and H. Bukhari et al., 2014. Identification of possible virulence marker from Campylobacter jejuni isolates. Emerging Infect. Dis., 20: 1026-1029.
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Asad, S., M. Hussain, A. Siddiqua, Q.U. Ain and H. Bokhari, 2014. Detection of molecular markers by comparative sequence analysis of enzymes from mycobacteria species. Pak. J. Bot., 46: 1065-1070.
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Sana, A., S.H. Mah-laka and H. Bokhari, 2013. Predictive mathematical modeling and statistical analysis for bacterial inactivation using microwave treatment. Pak. J. Public Health, 3: 27-30.
Khan, M.I., M. Ajmal, S. Micheal, M. Azam and A. Hussain et al., 2013. Homozygosity mapping identifies genetic defects in four consanguineous families with retinal dystrophy from Pakistan. Clin. Genet., 84: 290-293.
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Idrees, M., S.A.M.A.S. Eqani and H. Bokhari, 2013. Sol-gel immobilisation of methyl parathion degrading bacteria isolated from agricultural areas of Pakistan. Chem. Ecol., 29: 733-744.
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Eqani, S.A.M.A.S., R.N. Malik, A. Cincinelli, G. Zhang and A. Mohammad et al., 2013. Uptake of organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) by river water fish: the case of River Chenab. Sci. Total Environ., 450: 83-91.
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Bostan, N., N.E. Amen and H. Bokhari, 2013. Current and Future Prospects of Torque Teno Virus. J. Vaccines Vaccin., 10.4172/2157-7560.S1-004.
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Bokhari, H., M.A. Shah, S. Asad, S. Akhtar, M. Akram and B.W. Wren, 2013. Escherichia coli Pathotypes in Pakistan from Consecutive Floods in 2010 and 2011. Am. J. Trop. Med. Hyg., 88: 519-525.
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Syed, M.A., W. Ahmed, A. Naeem and H. Bokhari, 2012. Seroprevalence o f Bordetella pertussis antibodies to pertussis toxin among healthy children. J. Coll. Physicians Surg. Pak., 22: 677-678.
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Mughal, A., Y.F. Kazi, H.A. Bukhari and M. Ali, 2012. Pertussis resurgence among vaccinated children in Khairpur, Sindh, Pakistan. Public Health, 126: 518-522.
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Bokhari, H., I. Bilal and S. Zafar, 2012. BapC autotransporter protein of Bordetella pertussis is an adhesion factor. J. Basic Microbiol., 52: 390-396.
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Ajmal, M., M.I. Khan, S. Micheal, W. Ahmed and A. Shah et al., 2012. Identification of recurrent and novel mutations in TULP1 in Pakistani families with early-onset retinitis pigmentosa. Mol. Vision, 18: 1226-1237.
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Zafar, S., A. Nasir and H. Bokhari, 2011. Computational analysis reveals abundance of potential glycoproteins in Archaea, Bacteria and Eukarya. Bioinformation, 6: 352-355.
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Tabish, S., A. Raza, A. Nasir, S. Zafar and H. Bokhari, 2011. Analysis of glycosylation motifs and glycosyltransferases in Bacteria and Archaea Bioinformation, 6: 191-195.
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Syed, M.A., A.S. Bhatti, C. Li and H. Bokhari, 2011. Use of SPR biosensor for the study of proteolytic action of a serine protease enzyme. Am. J. Biomed. Sci., 3: 253-257.
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Syed, M.A. and S.H.A. Bokhari, 2011. Gold Nanoparticle Based Microbial Detection and Identification. J. Biomed. Nanotechnol., 7: 229-237.
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Raza, A., H. Shareef, H. Salim, R. Khushal and H. Bokhari, 2011. Selection of predicted siRNA as potential antiviral therapeutic agent against influenza virus. Bioinformation, 6: 340-343.
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Qureshi, M., A. Qadir, M., Rauf, A., Idrees and M., Mumtaz et al., 2011. Antimicrobial Efficacy of Metal-Barbiturate Conjugates Against Pathogenic Strains of Escherichia coli and Staphylococcus aureus. Lett. Drug Des. Discovery, 8: 980-987.
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Noofeli, M., H. Bokhari, P. Blackburn, M. Roberts, J.G. Coote and R. Parton, 2011. BapC autotransporter protein is a virulence determinant of Bordetella pertussis. Microb. Pathog., 51: 169-177.
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Muhammad, I., M. Uzma, B. Yasmin, Q. Mehmood and B. Habib, 2011. Prevalence of antimicrobial resistance and integrons in Escherichia Coli from Punjab, Pakistan. Braz. J. Microbiol., 42: 462-466.
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Mughal, A.A., Y.F. Kazi and S.H. Bukhari, 2011. Diagnosis of pertussis in vaccinated children of Khairpur, Sindh, Pakistan by Cough Plate Method. J. Microbiol. Infect. Dis., 1: 68-72.
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Idrees, M., M.A. Shah, S. Michael, R. Qamar and H. Bokhari, 2011. Antimicrobial Resistant Escherichia coli Strains Isolated From Food Animals in Pakistan. Pakistan J. Zool., 43: 303-310.
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Bokhari, H., M. Anwar, H.B. Mirza and P.M. Gillevet, 2011. Evidences of lateral gene transfer between archaea and pathogenic bacteria. bioinformation, 6: 293-296.
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Bokhari, H., F. Said, M.A. Syed, A. Mughal and Y.F. Kazi et al., 2011. Whooping cough in Pakistan: Bordetella pertussis vs Bordetella parapertussis in 2005-2009. Scand. J. Infect. Dis., 43: 818-820.
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Bokhari, H., F. Said, M.A. Syed, A. Mughal and Y.F. Kazi et al., 2011. Molecular typing of Bordetella parapertussis isolates circulating in Pakistan. FEMS Immunol. Med. Microbiol., 63: 373-380.
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Azam, M., R.W. Collin, A. Malik, M.I. Khan and S.T.A. Shah et al., 2011. Identification of Novel Mutations in Pakistani Families With Autosomal Recessive Retinitis Pigmentosa. Arch. Ophthalmol., 129: 1377-1378.
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Syed, M.A., U. Manzoor, I. Shah and S.H.A. Bukhari, 2010. Antibacterial effects of Tungsten nanoparticles on the Escherichia coli strains isolated from catheterized urinary tract infection (UTI) cases and Staphylococcus aureus. New Microbiol., 33: 329-335.
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Syed, M.A. and S.H. Bokhari, 2010. Adult and adolescent pertussis should not be underestimated. Pak. J. Med. Sci., 26: 244-245.
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Salman, A., A.T. Fard, A. Nasir and H. Bokhari, 2010. Comparative analysis of organophosphate degrading enzymes from diverse species. Bioinformation, 5: 67-72.
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Mirza, H.B., M. Anwar and S.H. Bokhari, 2010. In silico identification of potential horizontal gene transfer events between Archaea and pathogenic bacteria. J. Bioinf. Sequence Anal., 2: 36-41.
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Idress, M., U. Mussarat, Y. Badshah, R. Qamar and H. Bokhari, 2010. Virulence factors profile of drug-resistant Escherichia coli isolates from urinary tract infections in Punjab, Pakistan. Eur. J. Clin. Microbiol. Infect. Dis., 29: 1533-1537.
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Hashmi, T., S. Khan, Valeed, H. Bokhari, 2010. In silico identification of vaccine candidates against enteric pathogens by comparative genome sequence. Asian Pac. J. Mol. Biol. Biotechnol., 18: 327-331.
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Bokhari, H., C. Smith, K. Veerendra, J. Sivaraman, M. Sikaroodi and P. Gillevet, 2010. Novel fluorescent protein from Hydnophora rigida possess cyano emission. Biochem. Biophys. Res. Commun., 396: 631-636.
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Ajmal, M., W. Ahmed, A. Sadeque, S.H.B. Ali, S.H. Bokhari, N. Ahmed and R. Qamar, 2010. Identification of a recurrent insertion mutation in the LDLR gene in a Pakistani family with autosomal dominant hypercholesterolemia. Mol. Biol. Rep., 37: 3869-3875.
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Syed, M.A., S. Babar, A.S. Bhatti and H. Bokhari, 2009. Antibacterial effects of silver nanoparticles on the bacterial strains isolated from catheterized urinary tract infection cases. J. Biomed. Nanotechnol., 5: 209-214.
PubMed |
Syed, M.A., F. Said and S.H.A. Bukhari, 2009. Seroepidemiology of Bordetella pertussis infections in the twin cities of Pakistan. North Am. J. Med. Sci., 1: 353-355.
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Fard, A.T., A. Salman, B. Kazemi and H. Bokhari, 2009. In silico comparative genome analysis of malaria parasite Plasmodium falciparum and Plasmodium vivax chromosome 4. Parasitol. Res., 104: 1361-1364.
PubMed |
Azam, M., M.I. Khan, A. Gal, A. Hussain and S.T. Shah et al., 2009. A homozygous p.Glu150Lys mutation in the opsin gene of two Pakistani families with autosomal recessive retinitis pigmentosa. Mol. Vis., 15: 2526-2534.
PubMed |
Bokhari, S.H. and P. Blackburn, 2008. Purification and protective efficacy of re-natured C-terminal domain (P.30) of pertactin autotransporter protein of Bordetella pertussis. Pak. J. Bot., 40: 825-832.
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Bokhari, S.H. and P. Blackburn, 2008. Pertactin processing and secretion in protease(s) deficient strains of Escherichia coli suggestive of the possible role of proteases in folding of autotransporter proteins. Pak. J. Bot., 40: 815-824.
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Baig, S.M., A. Azhar, H. Hassan, J.M. Baig and A. Kiyani et al., 2006. Spectrum of beta-thalassemia mutations in various regions of Punjab and Islamabad, Pakistan: establishment of prenatal diagnosis. Haematologica, Vol. 91 http://www.haematologica.org/content/91/3/ELT02.full-text.pdf+html.
Baig, S.M., F. Rabbi, U. Hameed, J.A. Qureshi and Z. Mahmood et al., 2005. Molecular characterization of mutations causing β-thalassemia in Faisalabad Pakistan using the amplification refractory mutation system (ARMS-PCR). Indian J. Hum. Genet., 11: 80-83.
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