Prof. khaled Mohammad Khleifat
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Prof. khaled Mohammad Khleifat

Professor
Mutah University, Jordan

Highest Degree
Ph.D. in Biology from Illinois Institute of Technology, USA

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Biography

Professor Khaled M. Khleifat Gained his B.Sc. in Microbiology from Sallah Addin University, Irbil, Iraq, in 1988 and his MSc. in Biology from Ankara University in 1993. His PhD was in 1999 from Illinois Institute of Technology (IIT) Chicago, USA. After his PhD receiving, he has been appointed as an assistant professor at Mutah University in 1999. He has promoted to associate professor and full professor in august 7th 2004 and August 7th 2008, respectively. Prof. Khleifat was appointed several times in different academic positions lastly as the Vice Presedent for administrative and finential affairs since september, 2014 in Tafila Technical University, Jordan.
Generally, Khleifat is experienced in teaching Bio101 and 102, advanced microbial physiology, Microbial genetics, Molecular biology, environmental microbiology, cell biology, biochemistry and their labs.
Khleifat attended several national and international conferences and published more than 40 international peer reviewed articles. Khleifat’s research mostly focused on the environmental and medical microbiology and biotechnological areas: 1. isolation and identification of bacteria in different locations in south of Jordan., 2. Heavy metals uptake in bacteria, 3. bacterial hemoglobin biotechnology (effect on heavy metal bioaccumulation, enzyme productivity, response to antioxidants in bacteria). 4. bacterial composition of wastewater 5. Enzyme purification and kinetics. He had supervised more than 30 master students and 2 PhD students in the period between 2003-2015. He is a reviewer for several international peer-reviewd journals: Enzyme and Microbial Technology, Journal of Hazordous Materials, Current Microbiology, Biochemical Engineering Journal, Journal of Chemical Engineering, Mutah Lil-Buhuth Waddirasaat, Mutah University, Jordan, Journal of Basic Microbiology.
Dr. Khleifat-Khaled has received two major awards after receiving his PhD: (1) a one-year seniority for propmotion purpose due to his research achievements in his major, Microbiology and (2) Awarded Abdul-Hameed Shoman Prize for Young Arab Researchers for the Year 2009

Area of Interest:

Environmental Sciences
100%
Environmental Microbiology
62%
Micerobila Technology
90%
Microbial Physiology
75%
Environmental Biotechnology
55%

Research Publications in Numbers

Books
0
Chapters
0
Articles
0
Abstracts
0

Selected Publications

  1. Al-Limoun, M.O., K.M. Khleifat, K.Y. Alsharafa, H.N. Qaralleh and S.A. Alrawashdeh, 2019. Purification and characterization of a mesophilic organic solvent tolerant lipase produced by Acinetobacter sp. K5b4. Biocatal. Biotransformation, 37: 139-151.
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  2. Khleifat, K.M., M.O. Al-Limoun, K.Y. Alsharafa, H. Qaralleh and A.A. Al Tarawneh, 2018. Tendency of using different aromatic compounds as substrates by 2, 4-DNT dioxygenase expressed by pJS39 carrying the gene dntA from Burkholderia sp. strain DNT. Bioremed. J., (In Press) 10.1080/10889868.2018.1516619.
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  3. Al-Asoufi, A., A. Khlaifat, A. Tarawneh, K. Alsharafa, M. Al-Limoun and K. Khleifat, 2017. Bacterial quality of urinary tract infections in diabetic and non-diabetics of the population of Ma'an Province, Jordan. Pak. J. Biol. Sci., 20: 179-188.
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  4. Majali, I.S., S.A. Oran, K.M. Khleifat, H. Qaralleh, W. Rayyan and O.Y. Althunibat, 2016. Assessment of the antibacterial effects of Moringa peregrine extracts. Afr. J. Microbiol. Res., 9: 2410-2414.
  5. Althunibat, O.Y., Q. Qaralleh, S.Y.A. Al-Dalin, M. Abboud and K. Khleifat et al., 2016. Effect of thymol and carvacrol, the major components of Thymus capitatus on the growth of Pseudomonas aeruginosa. J. Pure Applied Microbiol., 10: 367-374.
  6. Khleifat, K.M., E.F. Sharaf and M.O. Al-Limoun, 2015. Biodegradation of 2-chlorobenzoic acid by Enterobacter cloacae: Growth kinetics and effect of growth conditions. Bioremed. J., 19: 207-217.
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  7. Allimoun, M., M.R. Ananzeh and K.M. Khleifat, 2015. Screening selection and optimization of extracellular methanol and ethanol tolerant lipase from Acinetobacter sp. K5b4. Int. J. Biosci., 6: 44-56.
  8. Khleifat, K.M., J. Alomari and A. Albadry, 2014. Prevalence and molecular diversity of Legionella pneumophila in domestic hot water systems of private apartments. Br. Microbiol. Res. J., 4: 306-316.
  9. Matar, S., S. Oran, K.M. Khleifat and R. Zeidan, 2013. Antimicrobial and antioxidant activities of leaf and fruit extracts of Jordanian Rubus sanguineus Friv. (Rosaceae). Afr. J. Microbiol., 7: 5114-5118.
  10. Tarawneh, K.A., Z.A. Halasah, A.M. Khleifat, M.I. Batarseh, K.M. Khleifat and A.H. Al-Mustafa, 2011. Evaluation of cefaclor oral suspensions stability using reversed phase high performance liquid chromatography and antimicrobial diffusion methods. Pak. J. Pharmaceut. Sci., 24: 1179-1190.
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  11. Tarawneh, K.A., F. Irshaid, A.S. Jaran, M. Ezealarab and K.M. Khleifat, 2010. Evaluation of antibacterial and antioxidant activities of methanolic extracts of some medicinal plants in northern part of Jordan. J. Biol. Sci., 10: 325-332.
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  12. Qaralleh, H., S. Idid, S. Saad, D. Susanti, M. Taher and K. Khleifat, 2010. [Antifungal and antibacterial activities of four malaysian sponge species (Petrosiidae)] Activite antifongique et antibacterienne de quatre eponges (Petrosiidae) isolees en Malaisie. J. Med. Mycol., 20: 315-320.
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  13. Khleifat, K.M., R.A. Halasah, K.A. Tarawneh, Z. Halasah, R. Shawabkeh and M.A. Wedyan, 2010. Biodegradation of linear alkylbenzene sulfonate by Burkholderia sp.: Effect of some growth conditions. Int. J. Agric. Biol., 12: 17-25.
  14. Khleifat, K.M., 2010. Characterization of 2, 4-dinitrotoluene dioxygenase from recombinant Esherichia coli strain PFJS39: Its direct interaction with Vitreoscilla hemoglobin. Bioremed. J., 14: 38-53.
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  15. Althunibat, O.Y., A.H. Al-Mustafa, K. Tarawneh, K.M. Khleifat, B.H. Ridzwan and H.N. Qaralleh, 2010. Protective role of Punica granatum L. peel extract against oxidative damage in experimental diabetic rats. Process Biochem., 45: 581-585.
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  16. Aljundi, I.H., K.M. Khleifat, A.M. Khlaifat, A.M. Ibrahim, K.A. Tarawneh and S.A. Tarawneh, 2010. Biodegradation of 2-chlorobenzoic acid by Klebsiella oxytoca: Mathematical modeling and effect of some growth conditions. Ind. Eng. Chem. Res., 49: 7159-7169.
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  17. Aljundi, I.H. and K.M. Khleifat, 2010. Biosorption of lead by E. coli strains expressingVitreoscilla hemoglobin: Isotherm modeling with two‐and three‐parameter models. Eng. Life Sci., 10: 225-232.
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  18. Abboud, M.M., I.H. Aljundi, K.M. Khleifat and S. Dmour, 2010. Biodegradation kinetics and modeling of whey lactose by bacterial hemoglobin VHb-expressing Escherichia coli strain. Biochem. Eng. J., 48: 166-172.
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  19. Tarawneh, K.A., N.M. Al‐Tawarah, A.H. Abdel‐Ghani, A.M. Al‐Majali and K.M. Khleifat, 2009. Characterization of verotoxigenic Escherichia coli (VTEC) isolates from faeces of small ruminants and environmental samples in Southern Jordan. J. Basic Microbiol., 49: 310-317.
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  20. Qaralleh, H.N., M.M. Abboud, K.M. Khleifat, K.A. Tarawneh and O.Y. Althunibat, 2009. Antibacterial activity in vitro of Thymus capitatus from Jordan. Pak. J. Pharmaceut. Sci., 22: 247-251.
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  21. Khleifat, K.M., K. Nawayseh, N.R. Adjeroud, A.M. Khlaifat, I.H. Aljundi and K.A. Tarawneh, 2009. Cadmium-resistance plasmid affected Cd+2 uptake more than Cd+2 adsorption in Klebsiella oxytoca. Bioremed. J., 13: 159-170.
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  22. Abboud, M., A. Al-Tarawneh, K.M. Khleifat, T. Khaled and H. Saed, 2009. Copper uptake in vivo and in vitro by Pseudomonas aeruginosa isolated from burn-infected patients. Curr. Microbiol., 59: 282-287.
  23. Tarawneh, K.A., S. Omar, O. Twissi, K.M. Khleifat, A. Al-Mustafa and K. Al-Sharafa, 2008. Antifungal and antioxidant effects of extracts of some medicinal plant species growing in south Jordan. Bull. Fac. Agric. Cairo Univ., 59: 242-249.
  24. Tarawneh, K.A., M. Wedyan, M.A. Al-zoubi, K.M. Khleifat and A. Tarawneh, 2008. Isolation and characterization of halophilic bacteria from the Dead Sea Coast, Jordan. Adv. Environ. Biol., 2: 63-69.
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  25. Tarawneh, K.A., K.M. Khleifat, A. Al-Mustafa, N. Alioui and M.A. Wedyan, 2008. Temporal expression of neurospora crassa tyrosinase gene under the control of glucose repressible gene-1 (grg-1) promoter. Aust. J. Basic Applied Sci., 2: 805-814.
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  26. Khleifat, K.M., K.A. Tarawneh, M.A. Wedyan, A.A. Al-Tarawneh and K. Al Sharafa, 2008. Growth kinetics and toxicity of Enterobacter cloacae grown on linear alkylbenzene sulfonate as sole carbon source. Curr. Microbiol., 57: 364-370.
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  27. Abboud, M.M., K.M. Khleifat, K.A. Tarawneh, A.H. Al-Mustafa, B.M. Elshafei and W. Al-Zereini, 2008. Effects of free amino acids on catechol oxidase from different plant sources. Adv. Food Sci., 30: 30-36.
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  28. Shawabkeh, R., K.M. Khleifat, I. Al-Majali and K. Tarawneh, 2007. Rate of biodegradation of phenol by Klebsiella oxytoca in minimal medium and nutrient broth conditions. Bioremed. J., 11: 13-19.
  29. Khleifat, K.M., I. Al-Majali, R. Shawabkeh and K. Tarawneh, 2007. Effect of carbon and nitrogen sources on the biodegradation of phenol by Klebsiella oxytoca and growth kinetic characteristics. Fresenius Environ. Bull., 16: 1-7.
  30. Khleifat, K.M., 2007. Effect of substrate adaptation, carbon starvation and cell density on the biodegradation of phenol by Actinobacillus sp. Fresenius Environ. Bull., 16: 726-730.
  31. Khleifat, K.M., 2007. Biodegradation of phenol by Actinobacillus sp.: Mathematical interpretation and effect of some growth conditions. Bioremed. J., 11: 103-112.
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  32. Khleifat, K.M. and A.H. Al-Mustafa, 2007. Effect of some nitrosative agents on the growth of vgb-bearing Enterobacter aerogenes strains. Curr. Microbiol., 55: 30-35.
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  33. Abboud, M.M., K.M. Khleifat, M. Batarseh, K.A. Tarawneh, A. Al-Mustafa and M. Al-Madadhah, 2007. Different optimization conditions required for enhancing the biodegradation of linear alkylbenzosulfonate and sodium dodecyl sulfate surfactants by novel consortium of Acinetobacter calcoaceticus and Pantoea agglomerans. Enzyme Microbial. Technol., 41: 432-439.
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  34. Khleifat, K.M., M.M. Abboud, S.S. Omar and J.H. Al-Kurishy, 2006. Urinary tract infection in South Jordanian population. J. Med. Sci., 6: 5-11.
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  35. Khleifat, K.M., M.M. Abboud, A.H. Al-Mustafa and K.Y. Al-Sharafa, 2006. Effects of carbon source and vitreoscilla hemoglobin (VHb) on the production of β-galactosidase in Enterobacter aerogenes. Curr. Microbiol., 53: 277-281.
  36. Khleifat, K.M., M.M. Abboud and A.H. Al-Mustafa, 2006. Effect of Vitreoscilla hemoglobin gene (vgb) and metabolic inhibitors on cadmium uptake by the heterologous host Enterobacter aerogenes. Process. Biochem., 41: 930-934.
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  37. Khleifat, K.M., M. Abboud, M. Laymun, K. Al-Sharafa and K. Tarawneh, 2006. Effect of variation in copper sources and growth conditions on the copper uptake by bacterial hemoglobin gene (vgb) bearing E. coli. Pak. J. Biol. Sci., 9: 2022-2031.
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  38. Khleifat, K.M., J. Alomari and A. Albadry, 2006. Biodegradation of Sodium Lauryl Ether Sulfate (SLES) by two different bacterial consortia. Curr. Microbiol., 53: 444-448.
  39. Khleifat, K.M., 2006. Correlation between bacterial hemoglobin and carbon sources: Their effect on copper uptake by transformed E. coli strain αDH5. Curr. Microbiol., 52: 64-68.
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  40. Khleifat, K.M., 2006. Biodegradation of phenol by Ewingella Americana: Effect of carbon starvation and some growth conditions. Process Biochem., 41: 2010-2016.
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  41. Khleifat, K., M. Abboud, W. Al-Shamayleh, A. Jiries and K.A. Tarawneh, 2006. Effect of chlorination treatment on gram negative bacterial composition of recycled wastewater. Pak. J. Biol. Sci., 9: 1660-1668.
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  42. Khleifat, K., 2006. Biodegradation of linear alkylbenzene sulfonate by a two-member facultative anaerobic bacterial consortium. Enz. Microbial. Technol., 3: 1030-1035.
  43. Shakhanbeh, J. and K. Khleifat, 2004. Failure of regeneration of sensory nerve fibers following neonatal denervation and crush lesion in rats. Turk. J. Biol., 27: 215-221.
  44. Khleifat, K. and M.M. Abboud, 2003. Correlation between bacterial hemoglobin gene (vgb) and aeration: Their effect on the growth and alph-amylase activity in transformed Enterobacter aerogenes. J. Applied Microbiol., 94: 1052-1058.
  45. Lahony, S.R., K. Khleifat, K.A. Al-Tarawneh, M.M. Abboud, R.M. Al-Oran, A.A. Al-Tarawneh and M.H. Homady, 2002. Variations in five species of Jordanian reptiles with new record on the land tortoises Testudo floweri (Testudinidae: Chelonia). Pak. J. Zool., 34: 43-49.
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  46. Khleifat, K., M.H. Homady and K.A. Tarawneh, 2002. Histopathological effects of Ferula hormonis extract on the colon of male mice. Mutah Lil-Buhuth wad-Dirasat, 17: 47-58.
  47. Khleifat, K., J. Shakhanbeh and K.A. Tarawneh, 2002. The chronic effects of Teucrium polium on some blood parameters and histopathology of liver and kidney in the rat. Turk. J. Biol., 26: 65-71.
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  48. Homady, M.H., K.M. Khleifat, K.A. Tarawneh and I.A. Al-Raheil, 2002. Reproductive toxicity and infertility effect of Ferula hormonis extracts in mice. Theriogenology, 57: 2247-2256.
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  49. Homady, M., H. Hussein, A. Jiries, A. Mahasneh, F. Al-Nasir and K. Khleifat, 2002. Survey of some heavy metals in sediments from vehicular service stations in Jordan and their effects on social aggression in prepubertal male mice. Environ. Res., 89: 43-49.
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  50. Khleifat, K.M., 2001. Presence of the bacterial hemoglobin gene (vgb) enhances culturability of a recombinant Escherichia coli αDH5 strain. J. Medical Sci., 1: 239-243.
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  51. Khleifat, K., M.H. Homady, K.A. Tarawneh and J. Shakhanbeh, 2001. Effect of Ferula hormonis extract on social aggression, fertility and some physiological parameters in prepubertal male mice. Endocrine. J., 48: 473-482.
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  52. Khliefat, K. and M.H. Homady, 2000. Bacterial hemoglobin gene (vgb) transformed into escherichia coli enhances lead-uptake and minimizes it's adsorption. Pak. J. Biol. Sci., 3: 1480-1483.
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