Prof. Dipesh  Dhakal
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Prof. Dipesh Dhakal

Professor
Sun Moon University, South Korea


Highest Degree
Ph.D. in Biochemistry from Sun Moon Univeristy, South Korea

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Biography

Mr. Dipesh Dhakal is currently working as Researcher/Executive Editor at Biotechnology Society of Nepal, Nepal. He has completed his M.Sc. in Biotechnology from Guru Nanak Dev University, Amritsar, India. His main area of interest focuses on Biomedical Sciences. His main area of expertise includes Microbiology, Antibiotics and Antibacterial, Molecular Biology, Microbial genetics, Biochemistry, Natural Products, Synthetic Biology, and Pharmaceutical Biotechnology. He has 7 publications in journals.

Area of Interest:

Pharmacology and Toxicology
100%
Pharmaceutical Sciences
62%
Medicinal Chemistry
90%
Molecular Biology
75%
Enzymology
55%

Research Publications in Numbers

Books
0
Chapters
0
Articles
0
Abstracts
0

Selected Publications

  1. Pokhrel, A.R., A.K. Chaudhary, H.T. Nguyen, D. Dhakal and T.T. Le et al., 2016. Overexpression of a pathway specific negative regulator enhances production of daunorubicin in bldA deficient Streptomyces peucetius ATCC 27952. Microbiol. Res., 192: 96-102.
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  2. Dhakal, D., A.K. Jha, A. Pokhrel, A. Shrestha and J.K. Sohng, 2016. Genetic manipulation of Nocardia species. Curr. Protocols Microbiol., Vol. 40. 10.1002/9780471729259.mc10f02s40.
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  3. Dhakal, D., A.K. Chaudhary, J.S. Yi, A.R. Pokhrel and B. Shrestha et al., 2016. Enhanced production of nargenicin A1 and creation of a novel derivative using a synthetic biology platform. Applied Microbiol. Biotechnol., 100: 9917-9931.
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  4. Pokhrel, A.R., D. Dhakal, A.K. Jha and J.K. Sohng, 2015. Herboxidiene biosynthesis, production, and structural modifications: Prospect for hybrids with related polyketide. Applied Microbiol. Biotechnol., 99: 8351-8362.
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  5. Jha, A.K., S. Paudel, D. Dhakal, P.T. Thuy Van, G.P. Ghimire and J.K. Sohng, 2015. Genetic evidence for the involvement of glycosyltransferase PdmQ and PdmS in biosynthesis of pradimicin from Actinomadura hibisca. Microbiol. Res., 174: 9-16.
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  6. Jha, A.K., D. Dhakal, P.T. Thuy Van, A.R. Pokhrel and T. Yamaguchi et al., 2015. Structural modification of herboxidiene by substrate-flexible cytochrome P450 and glycosyltransferase. Applied Microbiol. Biotechnol., 99: 3421-3431.
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  7. Dhakal, D., T.T. Le, R.P. Pandey, A.K. Jha and R.B. Gurung et al., 2015. Enhanced production of nargenicin A1 and generation of novel glycosylated derivatives. Applied Biochem. Biotechnol., 175: 2934-2949.
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  8. Dhakal, D. and J.K. Sohng, 2015. Laboratory maintenance of Nocardia species. Curr. Protoc. Microbiol., Vol. 39. 10.1002/9780471729259.mc10f01s39.
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  9. Dhakal, D. and J.K. Sohng, 2015. Commentary: Toward a new focus in antibiotic and drug discovery from the Streptomyces arsenal. Front. Microbiol., Vol. 6. 10.3389/fmicb.2015.00727.
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  10. Van, T.T., A.K. Jha, D. Dhakal, B. Shrestha and J.K. Sohng, 2014. Construction of recombinant vectors for metabolic engineering in Streptomyces species. J. Biomol. Reconstruct., 11: 55-60.
  11. Le, T.T., R.P. Pandey, R.B. Gurung, D. Dhakal and J.K. Sohng, 2014. Efficient enzymatic systems for synthesis of novel α-mangostin glycosides exhibiting antibacterial activity against Gram-positive bacteria. Applied Microbiol. Biotechnol., 98: 8527-8538.
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  12. Jha, A.K., T.T. Van, D. Dhakal, A.R. Pokhrel, B. Shrestha and J.K. Sohng, 2014. Deletion of LacI family transcriptional regulator herA involved in herboxidiene production in Streptomyces chromofuscus ATCC 49982. J. Biomol. Reconstruct., 11: 125-133.
  13. Jha, A.K., T.T. Van, D. Dhakal, A.R. Pokhrel, B. Shrestha and J.K. Sohng, 2014. Construction of clean host of Streptomyces chromofuscus ATCC 49982 for heterologous expression of gene cluster. J. Biomol. Reconstruct., 11: 115-124.
  14. Jha, A.K., T.T. Van, B. Shrestha, D. Dhakal and J.K. Sohng, 2014. Isolation and characterization of the expected 25-demethyl herboxidiene in medium No 6A6 from Streptomyces chromofuscus by mass analysis. J. Biomol. Reconstruct., 11: 30-35.
  15. Dhakal, D., T.L. Tuoi, A.R. Pokhrel, A.K. Jha, R.P. Pandey and J.K. Sohng, 2014. Enzymatic synthesis of glycosylated derivative of Nargenicin A1. J. Biomol. Reconstruct., 11: 9-15.
  16. Pokhrel, A.R., R.P. Pandey, P. Parajuli, A.K. Chaudhary, D. Dhakal and J.K. Sohng, 2013. An attempt to enhance the solubility of violacein generating glucoside analogs. J. Biomol. Reconstruct., 10: 100-106.
  17. Pokhrel, A.R., A.K. Chaudhary, D. Dhakal, A. Shrestha, Y.J. Yoon and J.K. Sohng, 2013. Cloning of two initial genes of synthetic epothilone biosynthetic gene cluster for transcript analysis and protein expression. J. Biomol. Reconstruct., 10: 65-73.
  18. Jha, A.K., B. Shrestha, D. Dhakal, H.C. Lee and J.K. Sohng, 2013. Enhancement of Spinosyns production in Saccharopolyspora spinosa NRRL 18395 (ATCC 83543.1) by metabolic engineering. J. Biomol. Reconstruct., 10: 27-35.
  19. Dhakal, D., A.K. Chaudhary, A. Pokhrel, A.K. Jha, S. Darsandhari, B. Shrestha and J.K. Sohng, 2013. Underpinning the secondary metabolites from Nocardia spp. J. Biomol. Reconstruct., 10: 9-17.
  20. Chaudhary, A.K., D. Dhakal and J.K. Sohng, 2013. An insight into the "-omics" based engineering of streptomycetes for secondary metabolite overproduction. BioMed Res. Int. 10.1155/2013/968518.
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  21. Chaudhary, A.K., A.R. Pokhrel, D. Dhakal and J.K. Sohng, 2013. Bioinformatic analysis of T1 pks-nrps, a PKS and NRPS hybrid pathways from complete genome of Streptomyces venenzulaeATCC 10712. J. Biomol. Reconstruct., 10: 1-8.
  22. Jha, A.K., D. Dhakal, H.C. Lee and J.K. Sohng, 2012. Enhancement of pradimicin production in Actinomadura hibisca P157-2 by optimization of carbon and nitrogen sources. J. Biomol. Reconstruct., 9: 103-107.
  23. Dinesh, K., D. Dhakal and J.K. Sohng, 2012. Effect of different biosynthetic precursors on the production of nargenicin A1 from metabolically engineered Nocardia sp. CS682. J. Microbiol. Biotechnol., 22: 1127-1132.
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  24. Dhakal, D., S. Bhattarai, K. Bhattarai, H.C. Lee and J.K. Sohng, 2012. Cloning and heterologous expression of pltA and couN6 in Nocardiasp CS682. J. Biomol. Reconstruct., 9: 8-14.
  25. Dhakal, D., A.K. Jha, S. Bhattarai, J.K. Sohng and K. Liou, 2012. In silico study of putative Vitamin D3 hydroxylase from Nocardia sp CS682. J. Biomol. Reconstruct., 9: 116-125.
  26. Koju, D., D. Dhakal, J.W. Park and J.K. Sohng, 2011. Prenylation of flavonoids by biotransformation of prenyltransferase SP5621 from Streptomyces peucetius ATCC27952. J. Biomol. Reconstruct., 8: 114-119.
  27. Gurung, R.B., D. Dhakal, T.T. Thuy and J.K. Sohng, 2011. Effect of change in composition of media on secondary metabolite production from doxorubicin gene cluster deleted-mutant of Streptomyces peucetius. J. Biomol. Reconstruct., 8: 71-76.
  28. Dhakal, D., D. Koju, H.C. Lee and J.K. Sohng, 2011. Study of plausible role of cytochrome P450 in nargenicin A1 biosynthesis from Nocardia sp. CS682 through disruption by homologous recombination. J. Biomol. Reconstruct., 8: 102-107.
  29. Thapa, R., D. Dhakal and D.P. Gauchan, 2007. Effect of different sugars on shoot induction in cv. Basmati. Kathmandu Univ. J. Sci. Eng. Technol., 1: 1-4.
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