Dr. Lim Boon Huat

Deputy Dean
School of Health Science, Malaysia

Highest Degree
Ph.D. in Molecular Medical Parasitology from University of Science, Malaysia

My URL
https://livedna.org/60.20361

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Area of Interest:

Animal and Veterinary Sciences
Infectious Disease
Molecular Biology
Parasite Ecology
Biochemistry

Selected Publications

  1. Wong, W.K., P.C. Foo, A. Olivos-Garcia, R. Noordin and Z. Mohamed et al., 2017. Parallel ELISAs using crude soluble antigen and excretory-secretory antigen for improved serodiagnosis of amoebic liver abscess. Acta Trop., 172: 208-212.
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  2. Foo, P.C., Y.Y. Chan, M. Mohamed, W.K. Wong, A.N. Najian and B.H. Lim, 2017. Development of a thermostabilised triplex LAMP assay with dry-reagent four target lateral flow dipstick for detection of Entamoeba histolytica and non-pathogenic Entamoeba spp. Analytica Chim. Acta, 966: 71-80.
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  3. Wong, W.K., P.C. Foo, M.N.M. Roze, C.D. Pim, P. Subramaniam and B.H. Lim, 2016. Helminthic infection and nutritional studies among orang asli children in Sekolah Kebangsaan Pos Legap, Perak. Can. J. Infect. Dis. Med. Microbiol., Vol. 2016. 10.1155/2016/1326085.
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  4. Wong, W.K., B.H. Lim, J.M. Hamid Jan, P.C. Foo, J.F. Dony, M. Zeehaida and R. Noordin, 2016. Prevalence and distribution of anti-amoebic IgG antibody among orang Asli (Aborigines) in peninsular Malaysia. Trop. Biomed., 33: 739-745.

  5. Ujang, J.A., S.H. Kwan, M.N. Ismail, B.H. Lim, R. Noordin and N. Othman, 2016. Proteome analysis of excretory-secretory proteins of Entamoeba histolytica HM1: IMSS via LC-ESI-MS/MS and LC-MALDI-TOF/TOF. Clin. Proteomics, Vol. 13. 10.1186/s12014-016-9135-8.
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  6. Abdelkareem, E.A., B.H. Lim, A.H. Shariet and B.Y. Khoo, 2015. Silencing of IL-21 on HT29 and HCT116 cells and determining its possible role in the proliferation of colorectal cancer associated with Schistosoma mansoni infection. Aust. J. Basic Applied Sci., 9: 39-44.

  7. Saidin, S., M.H. Yunus, N.D. Zakaria, K.A. Razak, L.B. Huat, N. Othman and R. Noordin, 2014. Production of recombinant Entamoeba histolytica pyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess. BMC Infect. Dis., Vol. 14. 10.1186/1471-2334-14-182.
    CrossRef  |  

  8. Huat, L.B., A.O. Garcia, T.Z. Ning, W.W. Kin and R. Noordin et al., 2014. Entamoeba histolytica acetyl-CoA synthetase: Biomarker of acute amoebic liver abscess. Asian Pacific J. Trop. Biomed., 4: 446-450.
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  9. Abdelkareem, E.A., T.G. Cheong, A.H. Sharief, L.B. Huat and K.B. Yin, 2014. Identification of specific proteins in colorectal cancer patients with Schistosoma mansoni infection as a possible biomarker for the treatment of this infection. Asian Pacific J. Trop. Dis., 4: S720-S724.
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  10. Tan, Z.N., W.K. Wong, R. Noordin, M. Zeehaida, G.A. Olivos and B.H. Lim, 2013. Efficacies of two in-house indirect ELISAs for diagnosis of amoebic liver abscess. Trop. Biomed., 30: 250-256.

  11. Othman, N., Z. Mohamed, M.M. Yahya, V.M. Leow, B.H. Lim and R. Noordin, 2013. Entamoeba histolytica antigenic protein detected in pus aspirates from patients with amoebic liver abscess. Exp. Parasitol., 134: 504-510.
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  12. Ning, T.Z., W.W. Kin, R. Noordin, S.T.W. Cun and F.P. Chong et al., 2013. Evaluation of Entamoeba histolytica recombinant phosphoglucomutase protein for serodiagnosis of amoebic liver abscess. BMC Infect. Dis., Vol. 13. 10.1186/1471-2334-13-144.
    CrossRef  |  

  13. Huat, L.B., 2013. Discovery of Trichuris landak n. sp. by Endang Purwaningsih. Asian Pacific J. Trop. Biomed., Vol. 3. 10.1016/S2221-1691(13)60070-2.
    CrossRef  |  

  14. Ning, T.Z., W.W. Kin, S. Mustafa, A. Ahmed and R. Noordin et al., 2012. Detection of Entamoeba histolytica in experimentally induced amoebic liver abscess: Comparison of three staining methods. Asian Pacific J. Trop. Biomed., 2: 61-65.
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  15. Huat, L.B., A.K. Mitra, N.I.N. Jamil, P.C. Dam, H.J.J. Mohamed and W.A.M.W. Muda, 2012. Prevalence and risk factors of intestinal helminth infection among rural Malay children. J. Global Infect. Dis., 4: 10-14.
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  16. Foo, P.C., Y.Y. Chan, W.C.S. Too, Z.N. Tan, W.K. Wong, P. Lalitha and B.H. Lim, 2012. Development of a thermostabilized, one-step, nested, tetraplex PCR assay for simultaneous identification and differentiation of Entamoeba species, Entamoeba histolytica and Entamoeba dispar from stool samples. J. Med. Microbiol., 61: 1219-1225.
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  17. Wong, W.K., Z.N. Tan, N. Othman, B.H. Lim, Z. Mohamed, A.O. Garcia and R. Noordin, 2011. Analysis of Entamoeba histolytica excretory-secretory antigen and identification of a new potential diagnostic marker. Clin. Vaccine Immunol., 18: 1913-1917.
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  18. Wong, W.K., Z.N. Tan, B.H. Lim, Z. Mohamed, A. Olivos-Garcia and R. Noordin, 2011. Comparison of protein-free defined media and effect of l-cysteine and ascorbic acid supplementation on viability of axenic Entamoeba histolytica. Parasitol. Res., 108: 425-430.
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  19. Chua, A.L., H.T. Elina, B.H. Lim, C.Y. Yean, M. Ravichandran and P. Lalitha, 2011. Development of a dry reagent-based triplex PCR for the detection of toxigenic and non-toxigenic Vibrio cholerae. J. Med. Microbiol., 60: 481-485.
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  20. Chua, A., C.Y. Yean, M. Ravichandran, B. Lim and P. Lalitha, 2011. A rapid DNA biosensor for the molecular diagnosis of infectious disease. Biosensors Bioelectronics, 26: 3825-3831.
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  21. Tan, Z.N., W.K. Wong, Z. Nik Zairi, B. Abdullah and N. Rahmah et al., 2010. Identification of Entamoeba histolytica trophozoites in fresh stool sample: Comparison of three staining techniques and study on the viability period of the trophozoites. Trop. Biomed., 27: 79-88.

  22. Othman, N., Z. Mohamed, J.J. Verweij, L.B. Huat, A. Olivos-Garcia, C. Yeng and R. Noordin, 2010. Application of real-time polymerase chain reaction in detection of Entamoeba histolytica in pus aspirates of liver abscess patients. Foodborne Pathogens Dis., 7: 637-641.
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  23. Zeehaida, M., N.Z. Zairi, Z.N. Tan, W.K. Wong and B.H. Lim, 2009. Research Note Seroprevalence of anti-amoebic antibody among blood donors by indirect hemeagglutination assay. Trop. Biomed., 26: 366-368.
    Direct Link  |  

  24. Rahmah, N., B.H. Lim, R. Mehdi and M.N. Nazmi, 2005. Treatment follow-up of Brugia malayi microfilaraemic and amicrofilaraemic individuals with serological evidence of active infection. Malaysian J. Microbiol., 1: 42-47.
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  25. Noordin, R., S. Wahyuni, A. Mangali, L.B. Huat, M. Yazdanbakhsh and E. Sartono, 2004. Comparison of IgG4 assays using whole parasite extract and BmR1 recombinant antigen in determining antibody prevalence in brugian filariasis. Filaria J., Vol. 3. 10.1186/1475-2883-3-8.
    CrossRef  |  

  26. Lim, B.H., R. Noordin, Z.M. Nor, R.A. Rahman, K.A. Abdullah and S. Sinnadurai, 2004. Brugia malayi infection in Meriones unguiculatus: Antibody response to recombinant BmR1. Exp. Parasitol., 108: 1-6.
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  27. Rahmah, N., B.H. Lim, H. Azian, T.S. Ramelah and A.R. Rohana, 2003. Use of a recombinant antigen‐based ELISA to determine prevalence of brugian filariasis among Malaysian schoolchildren near Pasir Mas, Kelantan-Thailand border. Trop. Med. Int. Health, 8: 158-163.
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  28. Lim, B.H., N. Rahmah, M. Ismail and K.A. Al-Salihi, 2002. Confocal laser scanning microscope observation on immunolocalization of native protein of BmR1 in adult Brugia malayi. Ann. Microscopy, 3: 114-116.

  29. Rahmah, N., S. Taniawati, R.K. Shenoy, B.H. Lim and V. Kumaraswami et al., 2001. Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection. Trans. Royal Soc. Trop. Med. Hygiene, 95: 601-604.
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  30. Rahmah, N., B.H. Lim, A.K. Anuar, R.K. Shenoy and V. Kumaraswami et al., 2001. A recombinant antigen-based IgG4 ELISA for the specific and sensitive detection of Brugia malayi infection. Trans. Royal Soc. Trop. Med. Hygiene, 95: 280-284.
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  31. Lim, B.H., N. Rahmah, S.A. Afifi, A. Ramli and R. Mehdi, 2001. Comparison of Brugia-Elisa and thick blood smear examination in a prevalence study of brugian filariasis in Setiu, Terengganu, Malaysia. Med. J. Malaysia, 56: 491-496.
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  32. Rahmah, N., A.K. Anuar, A.N. A'shikin, B.H. Lim, R. Mehdi, B. Abdullah and M.N. Zurainee, 1998. A Brugia malayi antigen specifically recognized by infected individuals. Biochem. Biophys. Res. Commun., 250: 586-588.
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  33. Lim, B.H., A.K. Anuar and N. Rahmah, 1995. Production and characterization of monoclonal antibody against 30 kDa antigen of Toxoplasma gondii. Trop. Biomed., 12: 5-11.
Member since October, 2017