Prof. Dr. Cecilia  R.C. Calado
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Prof. Dr. Cecilia R.C. Calado

Adjunct Professor
High Institute of Engineering of Lisbon, Portugal


Highest Degree
Ph.D. in Biotechnology from Technical University of Lisbon, Portugal

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Biography

Cecília R.C. Calado has a PhD in Biotechnology, focusing the Biopharmaceutical Process Engineering,from the Instituto Superior Técnico (IST),Technical University of Lisbon,a MSc in Biochemical Engineering from IST and a Biochemistry (5 years) degree from the Sciences Faculty from the Lisbon University.Cecília Calado presents a broad experience in R&Di in Biopharmaceutical Process Engineering and Discovery and Development of New Drugs.Cecília Calado has coordinated scientific projects,financed by agencies external to the University in a total of 1 378 000€. She is responsible for the research laboratory in Biomedical/ Pharmaceutical Engineering at High Institute of Engineering of Lisbon (ISEL – Instituto Superior de Engenharia de Lisboa, Instituto Politécnico de Lisboa). Cecília Calado is Professor at ISEL, were coordinates the BSc and the MSc in Biomedical Engineering.She evaluated scientific projects at CRUP (Council of Deans of the Portuguese Universities), of the
BES Contest and in 2011 at the Cyprus Research Promotion Foundation. Simultaneously to these multiple projects,she has promoted various activities to enhance public awareness to Science, such as Presentations on Patents and Technology Transfer.Between 2010 and Mars 2014, she was a member of the Executive Board of the Portuguese Chapter in Engineering in Medicine and Biology Society of IEEE. She a member of the IEEE, of the European Federation of Biotechnology, for Pharma and Medical Biotechnology and for Biochemical Engineering Science. She was member of 4 organizing and scientific boards of EMBS-IEEE Conferences - Portuguese Chapter and once of CIRP Conferences.Results driven, Cecília Calado believes in team play and entrepreneurship. Therefore,she led the HelicoVax Team of the 2012 Cohitec Program, to evaluate the creation of a hi-tech start-up.

Area of Interest:

Biomedical Sciences
100%
Bioseparation
62%
Biotechnology
90%
Microbiology
75%
Enzymology
55%

Research Publications in Numbers

Books
0
Chapters
0
Articles
31
Abstracts
0

Selected Publications

  1. Sampaio, P.N., K.C. Sales, F. Rosa, M.B. Lopes and C.R.C. Calado, 2016. High-throughput FTIR-based bioprocess analysis of recombinant cyprosin production. J. Ind. Microbiol. Biotechnol., 44: 49-61.
    CrossRef  |  Direct Link  |  
  2. Sales, K.C., F. Rosa, B.R. Cunha, P.N. Sampaio, M.B. Lopes and C.R. Calado, 2016. Metabolic profiling of recombinant Escherichia coli cultivations based on high‐throughput FT‐MIR spectroscopic analysis. Biotechnol. Prog., .
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  3. Rosa, F., K.C. Sales, J.G. Carmelo, A. Fernandes Platzgummer, C.L. da Silva, M.B. Lopes and C.R. Calado, 2016. Monitoring the ex‐vivo expansion of human mesenchymal stem/stromal cells in xeno‐free microcarrier‐based reactor systems by MIR spectroscopy. Biotechnol. Prog., 32: 447-455.
    Direct Link  |  
  4. Lopes, M., C.R.C. Calado, M. Figeiredo and J. Dias Bioucas, 2016. Does nonlinear modeling play a role in plasmid bioprocess monitoring based on Fourier transform infrared spectra? Appl. Spectrosc., .
  5. Sales, K.C., F. Rosa, P.N. Sampaio, L.P. Fonseca, M.B. Lopes and C.R. Calado, 2015. In situ near-infrared (NIR) versus high-throughput mid-infrared (MIR) spectroscopy to monitor biopharmaceutical production. Appl. Spectros., 69: 760-772.
    Direct Link  |  
  6. Rosa, F., K.C. Sales, B.R. Cunha, A. Couto, M.B. Lopes and C.R. Calado, 2015. A comprehensive high-throughput FTIR spectroscopy-based method for evaluating the transfection event: estimating the transfection efficiency and extracting associated metabolic responses. Anal. Bioanal. Chem., 407: 8097-8108.
    Direct Link  |  
  7. Lopes, M.B., G.A. Goncalves, D. Felicio Silva, K.L. Prather, G.A. Monteiro, D.M. Prazeres and C.R. Calado, 2015. In situ NIR spectroscopy monitoring of plasmid production processes: effect of producing strain, medium composition and the cultivation strategy. J. Chem. Technol. Biotechnol., 90: 255-261.
    Direct Link  |  
  8. Sampaio, P.N., K.C. Sales, F.O. Rosa, M.B. Lopes and C.R. Calado, 2014. In situ near infrared spectroscopy monitoring of cyprosin production by recombinant saccharomyces cerevisiae strains. J. Biotechnol., 188: 148-157.
    Direct Link  |  
  9. Lopes, M.B., G. Martins and C.R. Calado, 2014. Kinetic modeling of plasmid bioproduction in Escherichia coli DH5α cultures over different carbon-source compositions. J. Biotechnol., 186: 38-48.
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  10. Scholz, T., V.V. Lopes and C.R. Calado, 2012. High‐throughput analysis of the plasmid bioproduction process in escherichia coli by FTIR spectroscopy. Biotechnol. Bioeng., 109: 2279-2285.
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  11. Cadete, A., L. Figueiredo, R. Lopes, C.C.R. Calado, A.J. Almeida and L.M.D. Goncalves, 2012. Development and characterization of a new plasmid delivery system based on chitosan-sodium deoxycholate nanoparticles. Eur. J. Pharm. Sci., 45: 451-458.
    Direct Link  |  
  12. Vitoriano, I., A. Rocha Goncalves, T. Carvalho, M. Oleastro, C.R. Calado and M. Roxo Rosa, 2011. Antigenic diversity among Portuguese clinical isolates of Helicobacter pylori. Helicobacter, 16: 153-168.
    Direct Link  |  
  13. Sampaio, P.N., L. Sousa, C.R.C. Calado, M.S. Pais and L.P. Fonseca, 2011. Use of chemometrics in the selection of a saccharomyces cerevisiae expression system for recombinant cyprosin B production. Biotechnol. Lett., 33: 2111-2119.
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  14. Mendes, S., F. Camacho, T. Silva, C.R. Calado, A.C. Serra, A.M.D.A. Rocha Gonsalves and M. Roxo Rosa, 2011. A nonionic porphyrin as a noninterfering DNA antibacterial agent. Photochem. Photobiol., 87: 1395-1404.
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  15. Sampaio, P.N., C.R. Calado, L. Sousa, D.C. Bressler, M.S. Pais and L.P. Fonseca, 2010. Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production. Eur. Food Res. Technol., 231: 339-346.
    Direct Link  |  
  16. Silva, T., P. Lima, M. Roxo Rosa, S. Hageman, L.P. Fonseca and C.R.C. Calado, 2009. Prediction of dynamic plasmid production by recombinant escherichia coli fed-batch cultivations with a generalized regression neural network. Chem. Biochem. Eng. Q., 23: 419-427.
    Direct Link  |  
  17. Calado, C.R.C., M. Brandao, J. Biscaia, J.M.S. Cabral and L.P. Fonseca, 2009. Effect of tween-80 on stability and secretion of hydrophobic tagged-cutinases. Chem. Biochem. Eng. Q., 23: 411-417.
    Direct Link  |  
  18. Lienqueo, M.E., O. Salazar, C.R.C. Calado, L.P. Fonseca and J.M.S. Cabral, 2008. Influence of tryptophan tags on the purification of cutinase, secreted by a recombinant saccharomyces cerevisiae, using cationic expanded bed adsorption and hydrophobic interaction chromatography. Biotechnol. Lett., 30: 1353-1358.
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  19. Lienqueo, M.E., O. Salazar, K. Henriquez, C.R.C. Calado, L.P. Fonseca and J.M.S. Cabral, 2007. Prediction of retention time of cutinases tagged with hydrophobic peptides in hydrophobic interaction chromatography. J. Chromatogr. A, 1154: 460-463.
    Direct Link  |  
  20. Almeida, C.F., C.R. Calado, S.A. Bernardino, J. Cabral and L.P. Fonseca, 2006. A flow injection analysis system for on‐line monitoring of cutinase activity at outlet of an expanded bed adsorption column almost in real time. J. Chem. Technol. Biotechnol., 81: 1678-1684.
    Direct Link  |  
  21. Vojinovic, V., C.R. Calado, A.I. Silva, M. Mateus, J.M.S. Cabral and L.P. Fonseca, 2005. Micro-analytical GO/HRP bioreactor for glucose determination and bioprocess monitoring. Biosens. Bioelectron., 20: 1955-1961.
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  22. Ferreira, B.S., C.R. Calado, F. Van Keulen, L.P. Fonseca, J.M. Cabral and M.M. da Fonseca, 2004. Recombinant saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways. J. Biotechnol., 109: 159-167.
    Direct Link  |  
  23. Calado, C.R., B.S. Ferreira, M.M. da Fonseca, J.M. Cabral and L.P. Fonseca, 2004. Integration of the production and the purification processes of cutinase secreted by a recombinant Saccharomyces cerevisiae SU50 strain. J. Biotechnol., 109: 147-158.
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  24. Ferreira, B.S., C.R.C. Calado, F. Van Keulen, L.P. Fonseca, J.M.S. Cabral and M.M.R. Da Fonseca, 2003. Towards a cost effective strategy for cutinase production by a recombinant Saccharomyces cerevisiae: strain physiological aspects. Appl. Microbiol. Biotechnol., 61: 69-76.
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  25. Cunha, M.T., M.J.L. Costa, C.R.C. Calado, L.P. Fonseca, M.R. Aires Barros and J.M.S. Cabral, 2003. Integration of production and aqueous two-phase systems extraction of extracellular fusarium solani pisi cutinase fusion proteins. J. Biotechnol., 100: 55-64.
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  26. Calado, C.R., C. Almeida, J.M. Cabral and L.P. Fonseca, 2003. Development of a fed-batch cultivation strategy for the enhanced production and secretion of cutinase by a recombinant Saccharomyces cerevisiae SU50 strain. J. Biosci. Bioeng., 96: 141-148.
    Direct Link  |  
  27. Calado, C.R.C., M.A. Taipa, J.M.S. Cabral and L.P. Fonseca, 2002. Optimisation of culture conditions and characterisation of cutinase produced by recombinant Saccharomyces cerevisiae. Enzyme Microbial. Technol., 31: 161-170.
    CrossRef  |  Direct Link  |  
  28. Calado, C.R.C., J. Cabral and L.P. Fonseca, 2002. Effect of saccharomyces cerevisiae fermentation conditions on expanded bed adsorption of heterologous cutinase. J. Chem. Technol. Biotechnol., 77: 1231-1237.
    Direct Link  |  
  29. Calado, C.R., S.M. Monteiro, J.M. Cabral and L.P. Fonseca, 2002. Effect of pre-fermentation on the production of cutinase by a recombinant saccharomyces cerevisiae. J. Biosci. Bioeng., 93: 354-359.
    Direct Link  |  
  30. Calado, C.R., M. Mannesse, M. Egmond, J. Cabral and L.P. Fonseca, 2002. Production of wild‐type and peptide fusion cutinases by recombinant Saccharomyces cerevisiae MM01 strains. Biotechnol. Bioeng., 78: 692-698.
    CrossRef  |  
  31. Calado, C.R., G.E. Hamilton, J.M. Cabral, L.P. Fonseca and A. Lyddiatt, 2001. Direct product sequestration of a recombinant cutinase from batch fermentations of saccharomyces cerevisiae. Bioseparation, 10: 87-97.
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