1. Ribeiro da Cunha, B., L.P. Fonseca and C.R. Calado, 2019. Antibiotic discovery: Where have we come from, where do we go?. Antibiotics, Vol. 8. 10.3390/antibiotics8020045.
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2. Marques, V., B. Cunha, A. Couto, P. Sampaio, L.P. Fonseca, S. Aleixo and C.R. Calado, 2019. Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy. Spectrochimica Acta Part A: Mol. Biomol. Spectroscopy, 210: 193-202.
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3. Cunha, B., L.P. Fonseca and C.R.C. Calado, 2019. A phenotypic screening bioassay for Escherichia coli stress and antibiotic responses based on Fourier‐transform infrared (FTIR) spectroscopy and multivariate analysis. Applied Microbiol., 127: 1776-1789.
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4. Lopes, M.B. and C.R. Calado, 2018. Assessing plasmid bioprocess reproducibility and C‐source uptake stage through multivariate analysis of offline and online data. J. Chem. Technol. Biotechnol., 93: 3056-3066.
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5. Lopes, M., A. Amorim, C. Calado and P.R. Costa, 2018. Determination of cell abundances and paralytic shellfish toxins in cultures of the dinoflagellate gymnodinium catenatum by fourier transform near infrared spectroscopy. J. Marine Sci. Eng., Vol. 6. 10.3390/jmse6040147.
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6. Sampaio, P.N.S. and C.R.C. Calado, 2017. Comparative analysis of different transformed Saccharomyces cerevisiae strains based on high-throughput Fourier transform infrared spectroscopy. J. Biotechnol., 260: 1-10.
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7. Sales, K.C., F. Rosa, B.R. Cunha, P.N. Sampaio, M.B. Lopes and C.R. Calado, 2017. Metabolic profiling of recombinant Escherichia coli cultivations based on high‐throughput FT‐MIR spectroscopic analysis. Biotechnol. Progress, 33: 285-298.
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8. Lopes, M.B., C.R. Calado, M.A. Figueiredo and J.M. Bioucas-Dias, 2017. Does nonlinear modeling play a role in plasmid bioprocess monitoring using fourier transform infrared spectra?. Applied Spectroscopy, 71: 1148-1156.
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9. Sampaio, P.N., K.C. Sales, F. Rosa, M.B. Lopes and C.R.C. Calado, 2016. High-throughput FTIR-based bioprocess analysis of recombinant cyprosin production. J. Ind. Microbiol. Biotechnol., 44: 49-61.
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10. Rosa, F., K.C. Sales, J.G. Carmelo, A. Fernandes‐Platzgummer, C.L. da Silva, M.B. Lopes and C.R. Calado, 2016. Monitoring the ex‐vivo expansion of human mesenchymal stem/stromal cells in xeno‐free microcarrier‐based reactor systems by MIR spectroscopy. Biotechnol. Progress, 32: 447-455.
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11. Sales, K.C., F. Rosa, P.N. Sampaio, L.P. Fonseca, M.B. Lopes and C.R. Calado, 2015. In situ near-infrared (NIR) versus high-throughput mid-infrared (MIR) spectroscopy to monitor biopharmaceutical production. Appl. Spectros., 69: 760-772.
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12. Rosa, F., K.C. Sales, B.R. Cunha, A. Couto, M.B. Lopes and C.R. Calado, 2015. A comprehensive high-throughput FTIR spectroscopy-based method for evaluating the transfection event: estimating the transfection efficiency and extracting associated metabolic responses. Anal. Bioanal. Chem., 407: 8097-8108.
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13. Lopes, M.B., G.A. GonÒalves, D. FelØcio‐Silva, K.L. Prather, G.A. Monteiro, D.M. Prazeres and C.R. Calado, 2015. In situ NIR spectroscopy monitoring of plasmid production processes: effect of producing strain, medium composition and the cultivation strategy. J. Chem. Technol. Biotechnol., 90: 255-261.
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14. Sampaio, P.N., K.C. Sales, F.O. Rosa, M.B. Lopes and C.R. Calado, 2014. In situ near infrared spectroscopy monitoring of cyprosin production by recombinant saccharomyces cerevisiae strains. J. Biotechnol., 188: 148-157.
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15. Lopes, M.B., G. Martins and C.R.C. Calado, 2014. Kinetic modeling of plasmid bioproduction in Escherichia coli cultures conducted in different media compositions. J Biotechnol., 186: 38-48.
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16. Scholz, T., V.V. Lopes and C.R. Calado, 2012. High‐throughput analysis of the plasmid bioproduction process in Escherichia coli by FTIR spectroscopy. Biotechnol. Bioeng., 109: 2279-2285.
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17. Cadete, A., L. Figueiredo, R. Lopes, C.C.R. Calado, A.J. Almeida and L.M.D. Goncalves, 2012. Development and characterization of a new plasmid delivery system based on chitosan-sodium deoxycholate nanoparticles. Eur. J. Pharm. Sci., 45: 451-458.
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18. Vitoriano, I., A. Rocha Goncalves, T. Carvalho, M. Oleastro, C.R. Calado and M. Roxo Rosa, 2011. Antigenic diversity among Portuguese clinical isolates of Helicobacter pylori. Helicobacter, 16: 153-168.
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19. Sampaio, P.N., L. Sousa, C.R.C. Calado, M.S. Pais and L.P. Fonseca, 2011. Use of chemometrics in the selection of a saccharomyces cerevisiae expression system for recombinant cyprosin B production. Biotechnol. Lett., 33: 2111-2119.
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20. Mendes, S., F. Camacho, T. Silva, C.R. Calado, A.C. Serra, A.M.D.A. Rocha Gonsalves and M. Roxo Rosa, 2011. A nonionic porphyrin as a noninterfering DNA antibacterial agent. Photochem. Photobiol., 87: 1395-1404.
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21. Sampaio, P.N., C.R. Calado, L. Sousa, D.C. Bressler, M.S. Pais and L.P. Fonseca, 2010. Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production. Eur. Food Res. Technol., 231: 339-346.
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22. Silva, T., P. Lima, M. Roxo Rosa, S. Hageman, L.P. Fonseca and C.R.C. Calado, 2009. Prediction of dynamic plasmid production by recombinant escherichia coli fed-batch cultivations with a generalized regression neural network. Chem. Biochem. Eng. Q., 23: 419-427.
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23. Calado, C.R.C., M. Brandao, J. Biscaia, J.M.S. Cabral and L.P. Fonseca, 2009. Effect of tween-80 on stability and secretion of hydrophobic tagged-cutinases. Chem. Biochem. Eng. Q., 23: 411-417.
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24. Lienqueo, M.E., O. Salazar, C.R.C. Calado, L.P. Fonseca and J.M.S. Cabral, 2008. Influence of tryptophan tags on the purification of cutinase, secreted by a recombinant saccharomyces cerevisiae, using cationic expanded bed adsorption and hydrophobic interaction chromatography. Biotechnol. Lett., 30: 1353-1358.
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25. Lienqueo, M.E., O. Salazar, K. Henriquez, C.R.C. Calado, L.P. Fonseca and J.M.S. Cabral, 2007. Prediction of retention time of cutinases tagged with hydrophobic peptides in hydrophobic interaction chromatography. J. Chromatogr. A, 1154: 460-463.
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26. Almeida, C.F., C.R. Calado, S.A. Bernardino, J. Cabral and L.P. Fonseca, 2006. A flow injection analysis system for on‐line monitoring of cutinase activity at outlet of an expanded bed adsorption column almost in real time. J. Chem. Technol. Biotechnol., 81: 1678-1684.
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27. Vojinovic, V., C.R. Calado, A.I. Silva, M. Mateus, J.M.S. Cabral and L.P. Fonseca, 2005. Micro-analytical GO/HRP bioreactor for glucose determination and bioprocess monitoring. Biosens. Bioelectron., 20: 1955-1961.
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28. Ferreira, B.S., C.R. Calado, F. Van Keulen, L.P. Fonseca, J.M. Cabral and M.M. da Fonseca, 2004. Recombinant saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways. J. Biotechnol., 109: 159-167.
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29. Calado, C.R., B.S. Ferreira, M.M. da Fonseca, J.M. Cabral and L.P. Fonseca, 2004. Integration of the production and the purification processes of cutinase secreted by a recombinant Saccharomyces cerevisiae SU50 strain. J. Biotechnol., 109: 147-158.
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30. Ferreira, B.S., C.R.C. Calado, F.V. Keulen, L.P. Fonseca, J.M.S. Cabral and M.M.R. Da Fonseca, 2003. Towards a cost effective strategy for cutinase production by a recombinant Saccharomyces cerevisiae: Strain physiological aspects. Applied Microbial. Biotechnol., 61: 69-76.
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31. Cunha, M.T., M.J.L. Costa, C.R.C. Calado, L.P. Fonseca, M.R. Aires Barros and J.M.S. Cabral, 2003. Integration of production and aqueous two-phase systems extraction of extracellular fusarium solani pisi cutinase fusion proteins. J. Biotechnol., 100: 55-64.
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32. Calado, C.R., C. Almeida, J.M. Cabral and L.P. Fonseca, 2003. Development of a fed-batch cultivation strategy for the enhanced production and secretion of cutinase by a recombinant Saccharomyces cerevisiae SU50 strain. J. Biosci. Bioeng., 96: 141-148.
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33. Calado, C.R.C., M.A. Taipa, J.M.S. Cabral and L.P. Fonseca, 2002. Optimisation of culture conditions and characterisation of cutinase produced by recombinant Saccharomyces cerevisiae. Enzyme Microbial. Technol., 31: 161-170.
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34. Calado, C.R.C., J. Cabral and L.P. Fonseca, 2002. Effect of saccharomyces cerevisiae fermentation conditions on expanded bed adsorption of heterologous cutinase. J. Chem. Technol. Biotechnol., 77: 1231-1237.
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35. Calado, C.R., S.M. Monteiro, J.M. Cabral and L.P. Fonseca, 2002. Effect of pre-fermentation on the production of cutinase by a recombinant saccharomyces cerevisiae. J. Biosci. Bioeng., 93: 354-359.
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36. Calado, C.R., M. Mannesse, M. Egmond, J. Cabral and L.P. Fonseca, 2002. Production of wild‐type and peptide fusion cutinases by recombinant Saccharomyces cerevisiae MM01 strains. Biotechnol. Bioeng., 78: 692-698.
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37. Calado, C.R., G.E. Hamilton, J.M. Cabral, L.P. Fonseca and A. Lyddiatt, 2001. Direct product sequestration of a recombinant cutinase from batch fermentations of saccharomyces cerevisiae. Bioseparation, 10: 87-97.
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