Dr. Huang   Zhiwei

Dr. Huang Zhiwei

Associate Professor
National University of Singapore, Singapore


Highest Degree
Ph.D. in Biomedical Sciences from National University of Singapore, Singapore

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Area of Interest:

Physical Science Engineering
100%
Biotechnology
62%
Image Processing
90%
Sensors
75%
Bionanoscience
55%

Education

2010

Ph.D.

National University of Singapore, Singapore

Biomedical Sciences

Selected Publications

  1. Ye, Y., F. Xu, G. Wei, Y. Xu, D. Pu, L. Chen and Z. Huang, 2017. Scalable Fourier transform system for instantly structured illumination in lithography. Opt. Lett., 42: 1978-1981.
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  2. Ming, L.C., N.R. Gangodu, T. Loh, W. Zheng, J. Wang, K. Lin and H. Zhiwei, 2017. Real time near-infrared Raman spectroscopy for the diagnosis of nasopharyngeal cancer. Oncotarget, 8: 49443-49450.
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  3. Lin, K., W. Zheng, C.M. Lim and Z. Huang, 2017. Real-time in vivo diagnosis of nasopharyngeal carcinoma using rapid fiber-optic Raman spectroscopy. Theranostics, 7: 3517-3526.
    CrossRef  |  Direct Link  |  

  4. Bae, K., W. Zheng and Z. Huang, 2017. Quantitative assessment of spinal cord injury using circularly polarized coherent anti-Stokes Raman scattering microscopy. Applied Phys. Lett., Vol. 111, No. 6. 10.1063/1.4991792.
    CrossRef  |  Direct Link  |  

  5. Wang, Z., W. Zheng, S.C.Y. Hsu and Z. Huang, 2016. Optical diagnosis and characterization of dental caries with polarization-resolved hyperspectral stimulated Raman scattering microscopy. Biomed. Opt. Express, 7: 1284-1293.
    CrossRef  |  Direct Link  |  

  6. Wang, Z., W. Zheng, C.Y.S. Hsu and Z. Huang, 2016. Polarization-resolved hyperspectral stimulated Raman scattering microscopy for label-free biomolecular imaging of the tooth. Applied Phys. Lett., Vol. 108, No. 3. 10.1063/1.4939923.
    CrossRef  |  Direct Link  |  

  7. Wang, Z., W. Zheng and Z. Huang, 2016. Lock-in-detection-free line-scan stimulated Raman scattering microscopy for near video-rate Raman imaging. Opt. Lett., 41: 3960-3963.
    CrossRef  |  Direct Link  |  

  8. Wang, J., W. Zheng, K. Lin and Z. Huang, 2016. Integrated Mueller-matrix near-infrared imaging and point-wise spectroscopy improves colonic cancer detection. Biomed. Optics Express, 7: 1116-1126.
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  9. Wang, J., W. Zheng, K. Lin and Z. Huang, 2016. Development of a hybrid Raman spectroscopy and optical coherence tomography technique for real-time in vivo tissue measurements. Opt. Lett., 41: 3045-3048.
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  10. Wang, J., K. Lin, W. Zheng, K.Y. Ho, M. Teh, K.G. Yeoh and Z. Huang, 2016. Fiber-optic Raman spectroscopy for in vivo diagnosis of gastric dysplasia. Faraday Discussions, 187: 377-392.
    CrossRef  |  Direct Link  |  

  11. Lin, K., W. Zheng, C.M. Lim and Z. Huang, 2016. Real-time in vivo diagnosis of laryngeal carcinoma with rapid fiber-optic Raman spectroscopy. Biomed. Opt. Express, 7: 3705-3715.
    CrossRef  |  Direct Link  |  

  12. Lin, K., J. Wang, W. Zheng, K.Y. Ho, M. Teh, K.G. Yeoh and Z. Huang, 2016. Rapid fiber-optic Raman spectroscopy for real-time in vivo detection of gastric intestinal metaplasia during clinical gastroscopy. Cancer Prev. Res., 9: 476-483.
    CrossRef  |  Direct Link  |  

  13. Bergholt, M.S., K. Lin, J. Wang, W. Zheng and H. Xu et al., 2016. Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy enhances real-time in vivo diagnosis of adenomatous polyps during colonoscopy. J. Biophoton., 9: 333-342.
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  14. Wang, Z., W. Zheng, C.Y.S. Hsu and Z. Huang, 2015. Epi-detected quadruple-modal nonlinear optical microscopy for label-free imaging of the tooth. Applied Phys. Lett., Vol. 106, No. 3. 10.1063/1.4906447.
    CrossRef  |  Direct Link  |  

  15. Wang, J., K. Lin, W. Zheng, K.Y. Ho, M. Teh, K.G. Yeoh and Z. Huang, 2015. Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy improves in vivo diagnosis of esophageal squamous cell carcinoma at endoscopy. Scient. Rep., Vol. 5. 10.1038/srep12957.
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  16. Wang, J., K. Lin, W. Zheng, K.Y. Ho, M. Teh, K.G. Yeoh and Z. Huang, 2015. Comparative study of the endoscope-based bevelled and volume fiber-optic Raman probes for optical diagnosis of gastric dysplasia in vivo at endoscopy. Anal. Bioanal. Chem., 407: 8303-8310.
    CrossRef  |  Direct Link  |  

  17. Dong, Z., M. Asbahi, J. Lin, D. Zhu and Y.M. Wang et al., 2015. Second-harmonic generation from sub-5 nm gaps by directed self-assembly of nanoparticles onto template-stripped gold substrates. Nano Lett., 15: 5976-5981.
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  18. Bergholt, M.S., W. Zheng, K. Lin, J. Wang and H. Xu et al., 2015. Characterizing variability of in vivo Raman spectroscopic properties of different anatomical sites of normal colorectal tissue towards cancer diagnosis at colonoscopy. Anal. Chem., 87: 960-966.
    CrossRef  |  Direct Link  |  

  19. Lin, J., W. Zheng, Z. Wang and Z. Huang, 2014. Label-free three-dimensional imaging of cell nucleus using third-harmonic generation microscopy. Applied Phys. Lett., Vol. 105, No. 10. 10.1063/1.4895577.
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  20. Lin, J., S. Teh, W. Zheng, Z. Wang and Z. Huang, 2014. Multimodal nonlinear optical microscopic imaging provides new insights into acetowhitening mechanisms in live mammalian cells without labeling. Biomed. Opt. Express, 5: 3116-3122.
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  21. Duraipandian, S., J. Mo, W. Zheng and Z. Huang, 2014. Near-infrared Raman spectroscopy for assessing biochemical changes of cervical tissue associated with precarcinogenic transformation. Analyst, 139: 5379-5386.
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  22. Bergholt, M.S., W. Zheng, K.Y. Ho, M. The and K.G. Yeoh et al., 2014. Fiberoptic confocal Raman spectroscopy for real-time in vivo diagnosis of dysplasia in Barrett's esophagus. Gastroenterology, 146: 27-32.
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  23. Wang, J., M.S. Bergholt, W. Zheng and Z. Huang, 2013. Development of a beveled fiber-optic confocal Raman probe for enhancing in vivo epithelial tissue Raman measurements at endoscopy. Opt. Lett., 38: 2321-2323.
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  24. Upputuri, P.K., J. Lin, L. Gong, X.Y. Liu, H. Wang and Z. Huang, 2013. Circularly polarized coherent anti-Stokes Raman scattering microscopy. Opt. Lett., 38: 1262-1264.
    CrossRef  |  Direct Link  |  

  25. Lin, J., S. Pan, W. Zheng and Z. Huang, 2013. Polarization-resolved second-harmonic generation imaging for liver fibrosis assessment without labeling. Applied Phys. Lett., Vol. 103, No. 17. 10.1063/1.4826516.
    CrossRef  |  Direct Link  |  

  26. Lin, J., K.Z.J. Er, W. Zheng and Z. Huang, 2013. Radially polarized tip-enhanced near-field coherent anti-Stokes Raman scattering microscopy for vibrational nano-imaging. Applied Phys. Lett., Vol. 103, No. 8. 10.1063/1.4819236.
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  27. Duraipandian, S., W. Zheng, J. Ng, J.J. Low, A. Ilancheran and Z. Huang, 2013. Non-invasive analysis of hormonal variations and effect of postmenopausal Vagifem treatment on women using in vivo high wavenumber confocal Raman spectroscopy. Analyst, 138: 4120-4128.
    CrossRef  |  Direct Link  |  

  28. Duraipandian, S., W. Zheng, J. Ng, J.J. Low, A. Ilancheran and Z. Huang, 2013. Near-infrared-excited confocal Raman spectroscopy advances in vivo diagnosis of cervical precancer. J. Biomed. Opt., Vol. 18, No. 6. 10.1117/1.JBO.18.6.067007.
    CrossRef  |  Direct Link  |  

  29. Bergholt, M.S., W. Zheng, K.Y. Ho, M. The and K.G. Yeoh et al., 2013. Fiber-optic Raman spectroscopy probes gastric carcinogenesis in vivo at endoscopy. J. Biophoton., 6: 49-59.
    CrossRef  |  Direct Link  |  

  30. Bergholt, M.S., W. Zheng, K.Y. Ho, K.G. Yeoh and Z. Huang, 2013. Raman endoscopy for objective diagnosis of early cancer in the gastrointestinal system. J. Gastroint. Dig. Syst., Vol. S1. 10.4172/2161-069X.S1-008.
    CrossRef  |  Direct Link  |  

  31. Bergholt, M.S., W. Zheng and Z. Huang, 2013. Development of a multiplexing fingerprint and high wavenumber Raman spectroscopy technique for real-time in vivo tissue Raman measurements at endoscopy. J. Biomed. Opt., Vol. 18, No. 3. 10.1117/1.JBO.18.3.030502.
    CrossRef  |  Direct Link  |  

  32. Bergholt, M.S., S. Duraipandian, W. Zheng and Z. Huang, 2013. Multivariate reference technique for quantitative analysis of fiber-optic tissue Raman spectroscopy. Anal. Chem., 85: 11297-11303.
    CrossRef  |  Direct Link  |  

  33. Lin, K., D.L.P. Cheng and Z. Huang, 2012. Optical diagnosis of laryngeal cancer using high wavenumber Raman spectroscopy. Biosens. Bioelectron., 35: 213-217.
    CrossRef  |  Direct Link  |  

  34. Duraipandian, S., W. Zheng, J. Ng, J.J. Low, A. Ilancheran and Z. Huang, 2012. Simultaneous fingerprint and high-wavenumber confocal Raman spectroscopy enhances early detection of cervical precancer in vivo. Anal. Chem., 84: 5913-5919.
    CrossRef  |  Direct Link  |  

  35. Duraipandian, S., M.S. Bergholt, W. Zheng, K.Y. Ho and M. The et al., 2012. Real-time Raman spectroscopy for in vivo, online gastric cancer diagnosis during clinical endoscopic examination. J. Biomed. Opt., Vol. 17, No. 8. 10.1117/1.JBO.17.8.081418.
    CrossRef  |  Direct Link  |  

  36. Dai, M.Q., W. Zheng, Z. Huang and L.Y.L. Yung, 2012. Aqueous phase synthesis of widely tunable photoluminescence emission CdTe/CdS core/shell quantum dots under a totally ambient atmosphere. J. Mater. Chem., 22: 16336-16345.
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  37. Bergholt, M.S., W. Zheng and Z. Huang, 2012. Characterizing variability in in vivo Raman spectroscopic properties of different anatomical sites of normal tissue in the oral cavity. J. Raman Spectrosc., 43: 255-262.
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  38. Bergholt, M.S., K. Lin, W. Zheng, Z. Huang and D. Lau, 2012. In vivo, real-time, transnasal, image-guided Raman endoscopy: Defining spectral properties in the nasopharynx and larynx. J. Biomed. Opt., Vol. 17, No. 7. 10.1117/1.JBO.17.7.077002.
    CrossRef  |  Direct Link  |  

  39. Shao, X., W. Zheng and Z. Huang, 2011. Near-infrared autofluorescence spectroscopy for in vivo identification of hyperplastic and adenomatous polyps in the colon. Biosens. Bioelectron., 30: 118-122.
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  40. Shao, X., W. Zheng and Z. Huang, 2011. In vivo diagnosis of colonic precancer and cancer using near-infrared autofluorescence spectroscopy and biochemical modeling. J. Biomed. Opt., Vol. 16, No. 6. 10.1117/1.3589099.
    CrossRef  |  Direct Link  |  

  41. Lin, J., F. Lu, W. Zheng, S. Xu, D. Tai, H. Yu and Z. Huang, 2011. Assessment of liver steatosis and fibrosis in a bile duct ligation rat model using integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique. J. Biomed. Opt., Vol. 16, No. 11. 10.1117/1.3655353.
    CrossRef  |  Direct Link  |  

  42. Lin, B., M.S. Bergholt, D.P. Lau and Z. Huang, 2011. Diagnosis of early stage nasopharyngeal carcinoma using ultraviolet autofluorescence excitation-emission matrix spectroscopy and parallel factor analysis. Analyst, 136: 3896-3903.
    CrossRef  |  Direct Link  |  

  43. Lim, N.S.J., Z. Hamed, C.H. Yeow, C. Chan and Z. Huang, 2011. Early detection of biomolecular changes in disrupted porcine cartilage using polarized Raman spectroscopy. J. Biomed. Opt., Vol. 16, No. 1. 10.1117/1.3528006.
    CrossRef  |  Direct Link  |  

  44. Duraipandian, S., W. Zheng, J. Ng, J.J. Low, A. Ilancheran and Z. Huang, 2011. In vivo diagnosis of cervical precancer using Raman spectroscopy and genetic algorithm techniques. Analyst, 136: 4328-4336.
    CrossRef  |  Direct Link  |  

  45. Bergholt, M.S., W. Zheng, K. Lin, Z. Huang and K.Y. Ho et al., 2011. Characterizing variability in in vivo Raman spectra of different anatomical locations in the upper gastrointestinal tract toward cancer detection. J. Biomed. Opt., Vol. 16, No. 3. 10.1117/1.3556723.
    CrossRef  |  Direct Link  |  

  46. Bergholt, M.S., W. Zheng, K. Lin, K.Y. Ho and M. Teh et al., 2011. Combining near-infrared-excited autofluorescence and Raman spectroscopy improves in vivo diagnosis of gastric cancer. Biosens. Bioelectron., 26: 4104-4110.
    CrossRef  |  Direct Link  |  

  47. Bergholt, M.S., W. Zheng, K. Lin, K.Y. Ho and M. Teh et al., 2011. In vivo diagnosis of gastric cancer using Raman endoscopy and ant colony optimization techniques. Int. J. Cancer, 128: 2673-2680.
    CrossRef  |  Direct Link  |  

  48. Bergholt, M.S., W. Zheng, K. Lin, K.Y. Ho and M. Teh et al., 2011. In vivo diagnosis of esophageal cancer using image-guided Raman endoscopy and biomolecular modeling. Technol. Cancer Res. Treat., 10: 103-112.
    CrossRef  |  Direct Link  |  

  49. Yuen, C., W. Zheng and Z. Huang, 2010. Optimization of extinction efficiency of goldā€coated polystyrene bead substrates improves surface-enhanced Raman scattering effects by post-growth microwave heating treatment. J. Raman Spectrosc., 41: 374-380.
    CrossRef  |  Direct Link  |  

  50. Yuen, C., W. Zheng and Z. Huang, 2010. Low-level detection of anti-cancer drug in blood plasma using microwave-treated gold-polystyrene beads as surface-enhanced Raman scattering substrates. Biosens. Bioelectron., 26: 580-584.
    CrossRef  |  Direct Link  |  

  51. Teh, S.K., W. Zheng, K.Y. Ho, M. Teh, K.G. Yeoh and Z. Huang, 2010. Near-infrared Raman spectroscopy for optical diagnosis in the stomach: Identification of Helicobacter-pylori infection and intestinal metaplasia. Int. J. Cancer, 126: 1920-1927.
    CrossRef  |  Direct Link  |  

  52. Teh, S.K., W. Zheng, K.Y. Ho, M. Teh, K.G. Yeoh and Z. Huang, 2010. Near-infrared Raman spectroscopy for early diagnosis and typing of adenocarcinoma in the stomach. Br. J. Surg., 97: 550-557.
    CrossRef  |  Direct Link  |  

  53. Shao, X., W. Zheng and Z. Huang, 2010. Polarized near-infrared autofluorescence imaging combined with near-infrared diffuse reflectance imaging for improving colonic cancer detection. Opt. Express, 18: 24293-24300.
    CrossRef  |  Direct Link  |  

  54. Mo, J., W. Zheng and Z. Huang, 2010. Fiber-optic Raman probe couples ball lens for depth-selected Raman measurements of epithelial tissue. Biomed. Opt. Express, 1: 17-30.
    CrossRef  |  Direct Link  |  

  55. Lu, F., W. Zheng, J. Lin and Z. Huang, 2010. Integrated coherent anti-Stokes Raman scattering and multiphoton microscopy for biomolecular imaging using spectral filtering of a femtosecond laser. Applied Phys. Lett., Vol. 96, No. 13. 10.1063/1.3377905.
    CrossRef  |  Direct Link  |  

  56. Lin, K., W. Zheng and Z. Huang, 2010. Integrated autofluorescence endoscopic imaging and point-wise spectroscopy for real-time in vivo tissue measurements. J. Biomed. Opt., Vol. 15, No. 4. 10.1117/1.3475955.
    CrossRef  |  Direct Link  |  

  57. Lin, J., W. Zheng, H. Wang and Z. Huang, 2010. Effects of scatterers' sizes on near-field coherent anti-Stokes Raman scattering under tightly focused radially and linearly polarized light excitation. Opt. Express, 18: 10888-10895.
    CrossRef  |  Direct Link  |  

  58. Lin, J., F. Lu, W. Zheng and Z. Huang, 2010. Annular aperture-detected coherent anti-Stokes Raman scattering microscopy for high contrast vibrational imaging. Applied Phys. Lett., Vol. 97, No. 8. 10.1063/1.3483772.
    CrossRef  |  Direct Link  |  

  59. Li, H., W. Zheng and Z. Huang, 2010. Rapid near-infrared fluorescence excitation-emission matrix spectroscopy for multifluorophore characterization using an acousto-optic tunable filter technique. J. Biomed. Opt., Vol. 15, No. 2. 10.1117/1.3374051.
    CrossRef  |  Direct Link  |  

  60. Huang, Z., S.K. Teh, W. Zheng, K. Lin, K.Y. Ho, M. Teh and K.G. Yeoh, 2010. In vivo detection of epithelial neoplasia in the stomach using image-guided Raman endoscopy. Biosens. Bioelectron., 26: 383-389.
    CrossRef  |  Direct Link  |  

  61. Huang, Z., M.S. Bergholt, W. Zheng, K. Lin, K.Y. Ho and K.G. Yeoh, 2010. In vivo early diagnosis of gastric dysplasia using narrow-band image-guided Raman endoscopy. J. Biomed. Opt., Vol. 15, No. 3. 10.1117/1.3420115.
    CrossRef  |  Direct Link  |  

  62. Fang, C., F. Lu, W. Zheng and Z. Huang, 2010. Triple-frequency symmetric subtraction scheme for nonresonant background suppression in Coherent Anti-Stokes Raman Scattering (CARS) microscopy. Opt. Express, 18: 15714-15724.
    CrossRef  |  Direct Link  |  

  63. Bergholt, M.S., W. Zheng, K. Lin, K.Y. Ho and M. Teh et al., 2010. Raman endoscopy for in vivo differentiation between benign and malignant ulcers in the stomach. Analyst, 135: 3162-3168.
    CrossRef  |  Direct Link  |  

  64. Teh, S.K., W. Zheng, K.Y. Ho, M. Teh, K.G. Yeoh and Z. Huang, 2009. Near-infrared Raman spectroscopy for gastric precancer diagnosis. J. Raman Spectrosc., 40: 908-914.
    CrossRef  |  Direct Link  |  

  65. Teh, S.K., W. Zheng, D.P. Lau and Z. Huang, 2009. Spectroscopic diagnosis of laryngeal carcinoma using near-infrared Raman spectroscopy and random recursive partitioning ensemble techniques. Analyst, 134: 1232-1239.
    CrossRef  |  Direct Link  |  

  66. Mo, J., W. Zheng, J.J.H. Low, J. Ng, A. Ilancheran and Z. Huang, 2009. High wavenumber Raman spectroscopy for in vivo detection of cervical dysplasia. Anal. Chem., 81: 8908-8915.
    CrossRef  |  Direct Link  |  

  67. Lu, F., W. Zheng and Z. Huang, 2009. Coherent anti-Stokes Raman scattering microscopy using tightly focused radially polarized light. Opt. Lett., 34: 1870-1872.
    CrossRef  |  Direct Link  |  

  68. Lin, J., H. Wang, W. Zheng, F. Lu, C. Sheppard and H. Zhiwei, 2009. Numerical study of effects of light polarization, scatterer sizes and orientations on near-field coherent anti-Stokes Raman scattering microscopy. Opt. Express, 17: 2423-2434.
    CrossRef  |  Direct Link  |  

  69. Lin, J., F. Lu, H. Wang, W. Zheng, C.J. Sheppard and Z. Huang, 2009. Improved contrast radially polarized coherent anti-Stokes Raman scattering microscopy using annular aperture detection. Applied Phys. Lett., Vol. 95, No. 13. 10.1063/1.3240874.
    CrossRef  |  Direct Link  |  

  70. Huang, Z., S.K. Teh, W. Zheng, J. Mo and K. Lin et al., 2009. Integrated Raman spectroscopy and trimodal wide-field imaging techniques for real-time in vivo tissue Raman measurements at endoscopy. Opt. Lett., 34: 758-760.
    CrossRef  |  Direct Link  |  

  71. Yuen, C., W. Zheng and Z. Huang, 2008. Surface-enhanced Raman scattering: Principles, nanostructures, fabrications and biomedical applications. J. Innov. Opt. Health Sci., 1: 267-284.
    CrossRef  |  Direct Link  |  

  72. Yuen, C., W. Zheng and Z. Huang, 2008. Improving surface-enhanced Raman scattering effect using gold-coated hierarchical polystyrene bead substrates modified with postgrowth microwave treatment. J. Biomed. Opt., Vol. 13, No. 6. 10.1117/1.3050447.
    CrossRef  |  Direct Link  |  

  73. Widjaja, E., W. Zheng and Z. Huang, 2008. Classification of colonic tissues using near-infrared Raman spectroscopy and support vector machines. Int. J. Oncol., 32: 653-662.
    CrossRef  |  Direct Link  |  

  74. Teh, S.K., W. Zheng, K.Y. Ho, M. Teh, K.G. Yeoh and Z. Huang, 2008. Diagnostic potential of near-infrared Raman spectroscopy in the stomach: Differentiating dysplasia from normal tissue. Br. J. Cancer, 98: 457-465.
    CrossRef  |  Direct Link  |  

  75. Teh, S.K., W. Zheng, K.Y. Ho, M. Teh, K.G. Yeoh and Z. Huang, 2008. Diagnosis of gastric cancer using near-infrared Raman spectroscopy and classification and regression tree techniques. J. Biomed. Opt., Vol. 13, No. 3. 10.1117/1.2939406.
    CrossRef  |  Direct Link  |  

  76. Lu, F., W. Zheng, C. Sheppard and Z. Huang, 2008. Interferometric polarization coherent anti-Stokes Raman scattering (IP-CARS) microscopy. Opt. Lett. 33: 602-604.
    CrossRef  |  Direct Link  |  

  77. Lu, F., W. Zheng and Z. Huang, 2008. Phase-controlled polarization coherent anti-Stokes Raman scattering microscopy for high-sensitivity and high-contrast molecular imaging. J. Opt. Soc. Am. B, 25: 1907-1913.
    CrossRef  |  Direct Link  |  

  78. Lu, F., W. Zheng and Z. Huang, 2008. Heterodyne polarization coherent anti-Stokes Raman scattering microscopy. Applied Phys. Lett., Vol. 92, No. 12. 10.1063/1.2902298.
    CrossRef  |  Direct Link  |  

  79. Lu, F., W. Zheng and Z. Huang, 2008. Elliptically polarized coherent anti-Stokes Raman scattering microscopy. Opt. Lett., 33: 2842-2844.
    CrossRef  |  Direct Link  |  

  80. Jaillon, F., W. Zheng and Z. Huang, 2008. Half-ball lens couples a beveled fiber probe for depth-resolved spectroscopy: Monte Carlo simulations. Applied Opt., 47: 3152-3157.
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  81. Jaillon, F., W. Zheng and Z. Huang, 2008. Beveled fiber-optic probe couples a ball lens for improving depth-resolved fluorescence measurements of layered tissue: Monte Carlo simulations. Phys. Med. Biol., 53: 937-952.
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