Dr. Nguyen Hoang  Loc

Dr. Nguyen Hoang Loc

Professor and Head of Department
Hue University, Vitenam


Highest Degree
PostDoc Fellow in Plant Molecular Biology from Chonbuk National University, Republic of Korea

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Biography

Dr. Nguyen Hoang Loc is currently working as Professor and Head of Department of Biotechnology Affiliation College of Sciences, Hue University, Hue, Vietnam. He has completed his Postdoctoral Fellow in Plant Molecular Biology from Chonbuk National University, Republic of Korea. He also completed 15 research projects as team leader. His main area of research interest focuses on Expression of Genes Coding Subunit Antigens in Plants and Bacteria, Regulation of Gene Expression in the Pathways of secondary Metabolism in Plant Cells, and Production of Recombinant Protein/Enzyme. He has published 6 books, 91 research articles in journal contributed as author/co-author.

Area of Interest:

Molecular Sciences
100%
Gene Expression
62%
Gene Cloning
90%
Recombinant Proteins
75%
Tissue Culture
55%

Selected Publications

  1. Anh, N.H.T., T.N.T. An, N.T. Nho, V.T.V. Dung and N.H. Loc, 2016. Effect of salicylic acid on the biosynthesis of solasodine in cell suspension culture of Solanum hainanense Hance. Plant Cell Biotechnol. Mol. Biol., 17: 14-20.
    Direct Link  |  

  2. Loc, N.H., T. Ngoc, H.T. Quang, N.D. Huy and N.N. Luong, 2015. Cloning and expression of two genes coding endo-β-1,4-glucanases from Trichoderma asperellum PQ34 in Pichia pastoris. Chem. Pap., (In Press). 10.1515/chempap-2015-0210.
    CrossRef  |  

  3. Loc, N.H., N.V. Song, D.T. Long, T.G. Kim and M.S. Yang, 2015. In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit. J. Plant Biochem. Biotechnol., 24: 129-134.
    CrossRef  |  Direct Link  |  

  4. Huy, N.D., C.L. Nguyen, H.S. Park, N.H. Loc and M.S. Choi et al., 2015. Characterization of a novel manganese dependent endoglucanase belongs in GH family 5 from Phanerochaete chrysosporium. J. Biosci. Bioeng., (In Press). 10.1016/j.jbiosc.2015.06.009.
    CrossRef  |  PubMed  |  Direct Link  |  

  5. Giang, N.T., H.T. Quang, N.D. Huy, N.H.T. Anh, T.T.P. Lan and N.H. Loc, 2015. Effect of elicitor on asiaticoside accumulation in centella (Centella asiatica (L.) Urban) cells. VNU J. Sci., 31: 44-49.

  6. Anh, N.H.T., T.N.T. An, L.T.H. Thanh, V.T.V. Dung and N.T. Nho et al., 2015. Study on effect of methyl jasmonate and yeast extract on the growth and enhancement of solasodine biosynthesis in cell cultures of Solanum hainanense Hance. Vietnam J. Biotechnol., 13: 513-519.

  7. Loc, N.H., N.T.K. Quynh, H.T. Quang, T.T. Lan, L.M.T. Ngoc, D.T.B. Lan and P.T. Long, 2014. Enhancing expression of recombinant fimbrial adhesin K88 isolated of enterotoxigenic Escherichia coli from piglet. Vietnam J. Biol., 36: 120-125.

  8. Loc, N.H., N.H.T. Anh, L.T.M. Khuyen and T.N.T. An, 2014. Effects of yeast extract and methyl jasmonate on the enhancement of solasodine biosynthesis in cell cultures of Solanum hainanense Hance. J. BioSci. Biotechnol., 3: 1-6.
    Direct Link  |  

  9. Loc, N.H., D.T. Long, T.G. Kim and M.S. Yang, 2014. Expression of Escherichia coli heat-labile enterotoxin B subunit in transgenic tomato (Lycopersicon esculentum L.) fruit. Czech J. Genet. Plant Breed., 50: 26-31.
    Direct Link  |  

  10. Lan, D.T.B., T.T. Lan, L.Q. Viet, P.V. Quyet, H.T. Quang, N.H. Loc and P.T. Long, 2014. Cloning and expression of gene encoding P23 protein from Cryptosporidium parvum. J. BioSci. Biotechnol., 3: 189-193.
    Direct Link  |  

  11. Loc, N.H., T.T. Lan, H.T. Quang, D.T.B. Lan and P.T. Long, 2013. Cloning and expression of genes encoding F107-C and K88-1NT fimbrial proteins of enterotoxigenic Escherichia coli from piglets. Indian J. Microbiol., 53: 488-491.
    CrossRef  |  Direct Link  |  

  12. Loc, N.H., N.T.Q. Hoa, P.T.K. Cuc and H.T. Quang, 2013. Expression of chitinase (chi42) gene from Trichoderma asperellum in Saccharomyces cerevisiae. Ann. Biol. Res., 4: 15-19.
    Direct Link  |  

  13. Loc, N.H., H.T.T. Lien, D.V. Giap and H.T. Quang, 2013. Purification of recombinant neutral protease (NPRC10) by partitioning in aqueous two-phase systems. Eur. J. Exp. Biol., 3: 252-257.
    Direct Link  |  

  14. Loc, N.H., 2013. Production of Some Pharmaceutical Compounds by Plant Cell Culture and Transgenic Plants. Hue University Publishing House, Hue, Vietnam.

  15. Loc, N.H. and N.T.D. Nhat, 2013. Production of asiaticoside from centella (Centella asiatica L. Urban) cells in bioreactor. Asian Pac. J. Trop. Biomed., 3: 806-810.
    CrossRef  |  Direct Link  |  

  16. Quang, H.T., H.T.M. Hoa, N.T.L. Anh, T.T.B. Phuong and N.H. Loc, 2012. Study on genetic diversity of some pummelo varieties (Citrus grandis (L.) Osbeck) in Vietnam. Vietnam J. Biotechnol., 10: 977-984.

  17. Nguyen, C.L., T.N. Thao, N.D. Huy, H.T. Quang, N.V. Tung and N.H. Loc, 2012. Characterization of extracellular protease from Bacillus pumilus I7. Vietnam J. Biotechnol., 10: 825-831.

  18. Loc, N.H., N.S. Cong, D.V. Giap, N.T.Q. Hoa, H.T. Quang and N.D. Huy, 2012. Production of recombinant NPRC10 protease in 14-L fermentation scale. Eur. J. Exp. Biol., 2: 913-918.
    Direct Link  |  

  19. Loc, N.H., L.P. Thao, H.T. Quang, N.T.Q. Hoa and N.T. Giang, 2012. Purification of chitinase from Trichoderma asperellum SH16 by polyethylene glycol/potassium phosphate aqueous two-phase system. Vietnam J. Biotechnol., 10: 833-838.

  20. Loc, N.H., D.T. Long, P.Y. Nhi, H.T.K. Hong, T.G. Kim and M.S. Yang, 2012. Preliminary evaluation of transgenic tomato plants expressing Escherichia coli heat-labile toxin B subunit grown under in vivo condition. Ann. Biol. Res., 3: 2070-2073.
    Direct Link  |  

  21. Loc, N.H. and N.T. Giang, 2012. Effects of elicitors on the enhancement of asiaticoside biosynthesis in cell cultures of centella (Centella asiatica L. Urban). Chem. Pap., 66: 642-648.
    CrossRef  |  Direct Link  |  

  22. Tuan, V.C., V.D. Hoang and N.H. Loc, 2011. Cell suspension culture of zedoary (Curcuma zedoaria Roscoe). VNU Journal of Sci. Natural Sci. Technol., 27: 64-70.

  23. Quang, H.T., N.V. Trung, H.T.A. Van, L.T. Xuan and N.T. Mai et al., 2011. Study on genetic diversity of Thanh tra pummelo (Citrus grandis (L.) Osbeck) at Thua Thien Hue. Vietnam J. Biotechnol., 9: 681-688.

  24. Quang, H.T., L.T. Xuan, N.T. Mai, N.V. Trung, N.T.T. Lien, T.T.B. Phuong and N.H. Loc, 2011. Genetic variability of Thanh tra pummelo (Citrus grandis Osbeck) in Thua Thien Hue, Vietnam. Anal. Biol. Res., 2: 306-314.
    Direct Link  |  

  25. Loc, N.H., N.V. Song, N.Q.D. Tien, T.T. Minh, P.T.Q. Nga, T.G. Kim and M.S. Yang, 2011. Expression of the Escherichia coli heat-labile enterotoxin B subunit in transgenic watercress (Nasturtium officinale L.). Plant Cell Tissue Organ Culture, 105: 39-45.
    CrossRef  |  

  26. Loc, N.H., L.T. Thinh, M.S. Yang and T.G. Kim, 2011. High expression of cholera toxin B subunit in transgenic tomato (Lycopersicon esculentum L.) fruits. Biotechnol. Bioprocess Eng., 16: 576-580.
    CrossRef  |  

  27. Loc, N.H., H.T. Quang, N.B. Hung, N.D. Huy, T.T.B. Phuong and T.T.T. Ha, 2011. Trichoderma asperellum chi42 genes encode chitinase. Mycobiology, 39: 182-186.
    Direct Link  |  

  28. Loc, N.H., H.T. Quang, B.T.H. Lam and D.T.T. Trang, 2011. The effects of culture conditions on neutral protease (NPRC10) in a recombinant Escherichia coli BL21 (DE3). Anal. Biol. Res., 2: 474-485.
    Direct Link  |  

  29. Loc, N.H., D.V. Giap and H.T. Quang, 2011. Production of NPRC10 protease by recombinant Escherichia coli through submerged culture in 40-L fermenter. Ann. Biol. Res., 2: 62-68.
    Direct Link  |  

  30. Loc, N.H., 2011. Plant Tissue and Cell Culture: Concepts and Applications. Hue University Publishing House, Hue, Vietnam.

  31. Loc, N.H. and H.V. Kiet, 2011. Micropropagation of Solanum hainanense Hance. Anal. Biol. Res., 2: 394-398.
    Direct Link  |  

  32. Tuan, V.C. and N.H. Loc, 2010. Production of curcumin from zedoary (Curcuma zedoaria Roscoe) cells cultured in 10-liter bioreactor. Vietnamese J. Biotechnol., 8: 1459-1464.

  33. Thinh, L.T., T.G. Kim, M.S. Yang and N.H. Loc, 2010. Study of growth and development of CTB (cholera toxin B subunit) transgenic tomato. Vietnamese J. Biotechnol., 8: 1271-1278.

  34. Thinh, L.T., T.G. Kim, M.S. Yang and N.H. Loc, 2010. Expression of CTB (cholera toxin B subunit) in tomato (Lycopersicon esculentum L.). Vietnamese J. Biotechnol., 8: 1853-1858.

  35. Song, N.V., N.Q.D. Tien, T.T. Minh, P.T.Q. Nga, N.H. Loc, 2010. Development of in vitro regeneration system in watercress (Nasturtium officinale L.). Vietnamese J. Biotechnol., 8: 1181-1184.

  36. Quang, H.T., N.T.T. Hien, N.T.T. Lien, T.T.B. Phuong, N.H. Loc, 2010. Genetic variation of Thanh tra pummelo (Citrus grandis (L.) Osbeck) at Huong Tra, Thua Thien Hue. Vietnamese J. Biotechnol., 8: 213-220.

  37. Quang, H.T., H.T.A. Van, N.V. Trung, T.T.B. Phuong and N.H. Loc, 2010. Genetic variability of Thanh tra pummelo (Citrus grandis Osbeck) in Hue, Vietnam. Vietnamese J. Biotechnol., 8: 1333-1341.

  38. Nhi, P.Y., T.G. Kim, M.S. Yang and N.H. Loc, 2010. Expression of Escherichia coli heat-labile enterotoxin B subunit in transgenic tomato (Lycopersicon esculentum L.). Vietnamese J. Biotechnol., 8: 1279-1285.

  39. Loc, N.T.P., V.C. Tuan and N.H. Loc, 2010. Investigation on antibacterial activity of essential oil extracted from zedoary (Curcuma zedoaria Roscoe) cells cultured in 10-liter bioreactor. Vietnamese J. Biotechnol., 8: 1465-1471.

  40. Loc, N.H., N.V. Song, N.Q.D. Tien, T.T. Minh, P.T.Q. Nga, T.T.B. Phuong, K.T. Geum and Y.M. Sik, 2010. Expression of heat-labile enterotoxin B subunit from Escherichia coli in transgenic watercress (Nasturtium officinale L.). Vietnamese J. Biotechnol., 8: 1173-1179.

  41. Loc, N.H., N.T.T. Mien and D.T.B. Thuy, 2010. Purification of extracellular α-amylase from Bacillus subtilis by partitioning in polyethylene glycol/potassium phosphate aqueous two-phase system. Anal. Microbiol., 60: 623-628.
    CrossRef  |  

  42. Loc, N.H., N.T. Tam, 2010. Asiaticoside production from cell culture of centella (Centella asiatica L. Urban). Biotechnol. Bioprocess Eng., 15: 1065-1070.

  43. Loc, N.H., N.H.T. Anh, D.H.N. Binh, M.S. Yang and T.G. Kim, 2010. Production of glycoalkaloids from callus cultures of Solanum hainanense Hance. J. Plant Biotechnol., 37: 96-101.

  44. Loc, N.H., N.H. Bach, T.G. Kim and M.S. Yang, 2010. Tissue culture and expression of Escherichia coli heat-labile enterotoxin B subunit in transgenic Peperomia pellucida. Protein Expression Purif., 72: 82-86.
    PubMed  |  

  45. Loc, N.H. and N.T.T. An, 2010. Effect of culture medium on the growth and asiaticosdie accumulation of centella (Centella asiatica L. ) cells. Vietnamese J. Biotechnol., 8: 517-522.

  46. Loc, N.H. and L.T.H. Thanh, 2010. Solasodine production from cell culture of Solanum hainanense Hance. Biotechnol. Bioprocess Eng., 16: 581-586.
    CrossRef  |  

  47. Hung, N.B., H.T. Quang, T.T.T. Ha, T.N. Thao, T.T.D. Chau and N.H. Loc, 2010. Isolation and characterization of 42 kDa chitinase from Trichoderma asperellum. Vietnamese J. Biotechnol., 8: 1651-1657.

  48. Loc, N.H., V.C. Tuan, D.H.N. Binh, T.T.B. Phuong, T.G. Kim and M.S. Yang, 2009. Investigation on the accumulation of sesquiterpenes and polysaccharide from cells of zedoary (Curcuma zedoaria Roscoe) cultured in a 10-L bioreactor. Biotechnol. Bioprocess Eng., 14: 619-624.

  49. Loc, N.H., L.V. Dung and T.Q. Dung, 2009. Recombinant DNA Technology. Ho Chi Minh City National University Publishing House, Ho Chi Minh City, Vietnam.

  50. Thi, H.T.M., N.V. Nhan, T.T.T. Huong and N.H. Loc, 2008. Appication of techniques of molecular biology in diagnosis of fragile X syndrome. Vietnamese J. Med. Res., 58: 18-23.

  51. Quang, H.T., N.D. Huy, T.T.B. Phuong and N.H. Loc, 2008. Two dimensional electrophoresis of protein in drought-tolerant rice (Oryza sativa L.) lines selected from in vitro cultures. Vietnamese J. Biotechnol., 6: 439-443.

  52. Phuong, T.T.B., T.T.K. Thu and N.H. Loc, 2008. Micropropagation of polygonum multiflorum thumb. Vietnamese J. Biotechnol., 6: 889-895.

  53. Phuong, T.T.B., D.T.T. Trang, N.V. Song and N.H. Loc, 2008. Determining blast resistance gene pi-b in some rice (Oryza sativa L.) cultivars. Vietnamese J. Biotechnol., 6: 61-68.

  54. Loc, N.H., T.T.B. Phuong, N.V. Song, P.T.P. Nhi, T.T.T. Chi and D.T.T. Trang, 2008. Determining blast resistance gene pi-ta in some rice (Oryza sativa L.) cultivars by PCR technique. Vietnamese J. Biotechnol., 6: 221-226.

  55. Loc, N.H., N.V. Song, H.T. Quang, L.D. Dung, D.D. Loi, T.T.B. Phuong and D.T.B. Thuy, 2008. Cloning and expression of neutral protease gene from Bacillus subtilis. Vietnamese J. Biotechnol., 6: 963-970.

  56. Loc, N.H., L.T.N. Minh and D.T.B. Thuy, 2008. Partitioning of extracellular protease from Bacillus subtilis in PEG/potassium phosphate aqueous two-phase systems. Vietnamese J. Biotechnol., 6: 375-382.

  57. Loc, N.H., D.T.H. Diem, D.H.N. Binh, D.T. Huong, T.G. Kim and M.S. Yang, 2008. Isolation and characterization of antioxidation enzymes from cells of zedoary (Curcuma zedoaria Roscoe) cultured in a 5-L bioreactor. Mol. Biotechnol., 38: 81-87.
    CrossRef  |  

  58. Loc, N.H., D.H.N. Binh, P.D. Lam, P.T.A. Kim and T.T.B. Phuong, 2008. Study on the accumulation of asiaticoside in callus of Centella asiatica (L.) Urban. Vietnamese J. Biotechnol., 6: 873-881.

  59. Loc, N.H., T.T. Le and H.T.M. Thi, 2007. Molecular Biology. Hue University Publishing House, Hue, Vietnam.

  60. Loc, N.H., 2007. Introduction to Biotechnology. Hue University Publishing House, Hue, Vietnam.

  61. Loc, N.H., T.T.T. Ha and Y. Hirata, 2006. Effect of several factors on cell biomass production of zedoary (Curcuma zedoaria Roscoe) in bioreactor. Vietnamese J. Biotechnol., 4: 213-220.

  62. Loc, N.H., 2006. Cell Biotechnology. Hue University Publishing House, Hue, Vietnam.

  63. Loc, N.H., D.T. Duc, T.H. Kwon and M.S. Yang, 2005. Micropropagation of zedoary (Curcum zedoaria Roscoe) a valuable medicinal plant. Plant Cell Tissue Org. Cult., 81: 119-122.
    CrossRef  |  

  64. Lee, J.H., N.H. Loc, T.H. Kwon and M.S. Yang, 2004. Partioning of recombinant human granulocyte-macrophage colony stimulating factor from plant cell suspension culture in PEG/sodium phosphate aqueous two-phase system. Biotechnol. Bioprocess Eng., 9: 12-16.
    Direct Link  |  

  65. Kang, T.J., N.H. Loc, M.O. Jang and M.S. Yang, 2004. Modification of the cholera toxin B subunit coding sequence to enhance expression in plants. Mol. Breeding, 13: 143-153.
    CrossRef  |  

  66. Phuong, T.T.B., N.T.H. Phuong, N.H. Loc and N.H. Dong, 2003. Osmotic adjustment of rice callus. Vietnamese J. Agric. Rural Develop., 4: 414-416.

  67. Loc, N.H., T.T.B. Phuong, D.T.T. Thuy and N.H. Dong, 2003. Selection of drought-tolerant lines of sugarcane (Saccharum officinarum L.). Vietnamese J. Biol., 25: 112-118.

  68. Loc, N.H., T.G. Kim, S.M. Park, T.H. Kwon, D.H. Kim and M.S. Yang, 2003. Ordered differential display of mRNA from Nicotiana tabacum. Adv. Natural Sci., 4: 331-338.

  69. Kang, T.J., T.H. Kwon, T.G. Kim, N.H. Loc and M.S. Yang, 2003. Comparing constitutive promoters using CAT activity in transgenic tobacco plants. Mol. Cells, 16: 117-122.
    PubMed  |  

  70. Kang, T.J., N.H. Loc, M.O. Jang, Y.S. Jang, Y.S. Kim, J.E. Seo and M.S. Yang, 2003. Expression of the B subunit of E. coli heat-labile enterotoxin in the chloroplasts of plants and its characterization. Transgenic Res., 12: 683-691.
    PubMed  |  

  71. Kang, T.J., J.E. Seo, N.H. Loc and M.S. Yang, 2003. Herbicide resistance of tobacco chloroplast expressing the bar gene. Mol. Cells, 16: 60-66.
    PubMed  |  

  72. Phuong, T.T.B., T.N.G. Ai, N.H. Loc and N.H. Dong, 2002. Selection of drought-tolerant lines of rice (Oryza sativa L.). Vietnamese J. Agric. Rural Develop., 7: 579-580.

  73. Loc, N.H., T.T.B. Phuong, T.T.P. Lan, D.T.T. Thuy and N.H. Dong, 2002. Effect of mannitol on proline and glucose accumulation in relation to osmotic adjustment in sugarcane (Saccharum officinarum L.). Vietnamese J. Agric. Rural Develop., 8: 664-666.

  74. Loc, N.H., N. Anh, D.N. Ai, V.Q. Man and D.Q. Bang, 2001. Propagation of Camellia japonica by cutting. Vietnamese J. Biol., 23: 39-44.

  75. Phuong, T.T.B., H.V. Kiet, N.H. Loc and N.H. Dong, 2000. Effect of mannitol on the change of cell structure in rice (Oryza sativa L.) callus cultures. Vietnamese J. Genet. Appl., 3: 13-20.

  76. Loc, N.H., V.C. Tuan, P.C. Binh and L.T. Thinh, 2000. Effects of mannitol on proline and glucose accumulation in relation to osmotic adjustment in rice (Oryza sativa L.) callus cultures. Vietnamese J. Biol., 22: 96-100.

  77. Loc, N.H., V.C. Tuan, L.T. Thinh and C.D. Nguyen, 2000. Effect of mannitol on the dynamic of lectin accumulation in rice (Oryza sativa L.) callus cultures. Vietnamese J. Biol., 22: 92-95.

  78. Loc, N.H., 2000. Gene transfer and applications in gene therapy. Vietnamese J. Biol., 22: 82-91.

  79. Loc, N.H. and M.V. Pho, 2000. Preliminary data on the orchidaceae in thua thien hue and in vitro preservative studies for some species of this family. Vietnamese J. Biol., 22: 173-178.

  80. Pho, M.V. and N.H. Loc, 1998. The diversity of the species composition on orchid family (Orchidaceae Juss.) living in Bach Ma National Park. Vietnamese J. Biol., 20: 33-37.

  81. Loc, N.H., 1998. Gene transformation technology in higher plants (review). Vietnamese J. Biol., 20: 7-14.

  82. Loc, N.H. and M.V. Pho, 1998. Micropropagation of aerides odorata orchid plant. Vietnamese J. Biol., 20: 41-43.

  83. Loc, N.H. and L.V.T. Huan, 1998. Induction of polyploid allium fistulosum plants by callus culture. Vietnamese J. Biol., 20: 44-48.

  84. Loc, N.H. and H.T.K. Hong, 1998. Micropropagation of Hippeastrum reticulatum by axillary bud and bulb scale culture. Proc. NTSC Vietnam, 10: 85-89.

  85. Loc, N.H., N. Anh and T.A. Thien, 1997. Study on the micropropagation of aquilaria crassna pierre. Vietnamese J. Biol., 19: 49-50.

  86. Loc, N.H., T.T. Thu, L.T. Binh and L.T. Muoi, 1994. The analysis of NaCl-tolerant stability in selected tobacco lines. Proc. NCST Vietnam, 6: 47-56.

  87. Loc, N.H., T.T. Thu, L.T. Muoi and L.T. Binh, 1992. Modifications of biochemical properties of NaCl-tolerant and desiccation-tolerant tobacco lines in in vitro culture. Vietnamese J. Genet. Appl., 1: 35-39.

  88. Loc, N.H., L.T. Binh, L.T. Muoi and H.T.T. Ngoc, 1992. Study of desiccation tolerant ability of tobacco callus in in vitro culture. Vietnamese J. Biol., 14: 31-37.

  89. Loc, N.H., L.T. Binh and L.T. Muoi, 1992. Osmotic adjustment of tobacco callus. Proc. NCSR Vietnam, 4: 73-80.

  90. Loc, N.H., L.T. Binh and L.T. Muoi, 1991. Desiccation and salt stresses stimulate growth and regeneration of craterostigma plantagineum hochst in in vitro culture. Proc. NCSR Vietnam, 3: 92-99.

  91. Loc, N.H., L.T. Binh and L.T. Muoi, 1990. Selection of NaCl-tolerant tobacco lines from diploid and haploid callus cultures. Vietnamese J. Biol., 12: 7-15.