Dr. Sudarshan Kumar
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Dr. Sudarshan Kumar

Senior Scientist
National Dairy Research Institute, India

Highest Degree
Ph.D. in Animal Biotechnology from Indian Veterinary Research Institute, India

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Area of Interest:

Animal and Veterinary Sciences
100%
Animal Biotechnology
62%
Small Ruminants
90%
Animal Physiology
75%
Dairy Science
55%

Research Publications in Numbers

Books
1
Chapters
1
Articles
82
Abstracts
7

Selected Publications

  1. Shashikumar, N.G., R.K. Baithalu, S. Bathla, S.A. Ali and P. Rawat et al., 2018. Global proteomic analysis of water buffalo (Bubalus bubalis) saliva at different stages of estrous cycle using high throughput mass spectrometry. Theriogenology, 110: 52-60.
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  2. Lotfan, M., S.A. Ali, M.L. Yadav, S. Choudhary, M.K. Jena, S. Kumar and A.K. Mohanty, 2018. Genome-wide gene expression analysis of 45 days pregnant fetal cotyledons vis-a-vis non-pregnant caruncles in buffalo (Bubalus bubalis). Gene, 654: 127-137.
    CrossRef  |  Direct Link  |  
  3. Bisht, S., K.S. Singh, R. Choudhary, S. Kumar and S. Grover et al., 2018. Expression of fibronectin-binding protein of L. acidophilus NCFM and in vitro refolding to adhesion capable native-like protein from inclusion bodies. Protein Expression Purif., 145: 7-13.
    CrossRef  |  Direct Link  |  
  4. Singh, K.S., R. Choudhary, S. Bisht, S. Grover, S. Kumar, A.K. Mohanty and J.K. Kaushik, 2017. Expression of recombinant truncated domains of mucus-binding (Mub) protein of Lactobacillus plantarum in soluble and biologically active form. Protein Expression Purif., 135: 54-60.
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  5. Pragya, P., G. Kaur, S.A. Ali, S. Bhatla and P. Rawat et al., 2017. High-resolution mass spectrometry-based global proteomic analysis of probiotic strains Lactobacillus fermentum NCDC 400 and RS2. J. Proteomics, 152: 121-130.
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  6. Kaur, G., S.A. Ali, S. Pachauri, D. Malakar, J.K. Kaushik, A.K. Mohanty and S. Kumar, 2017. Buffalo leukemia inhibitory factor induces differentiation and dome-like secondary structures in COS-1 cells. Cytogenet. Genome Res. 10.1159/000465507.
    CrossRef  |  
  7. Choudhary, R., T.S. Goud, A. Kumar, A.K. Sharma and S.V. Singh et al., 2017. Heat stress induced adaptation in melanocytes is dependent on the level of melanin and reduction of apoptosis. J. Dermatol. Sci., 85: 250-252.
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  8. Ali, S.A., G. Kaur, J.K. Kaushik, D. Malakar, A.K. Mohanty and S. Kumar, 2017. Examination of pathways involved in Leukemia Inhibitory Factor (LIF)-induced cell growth arrest using label-free proteomics approach. J. Proteomics, 168: 37-52.
    CrossRef  |  Direct Link  |  
  9. Saugandhika, S., V. Sharma, H. Malik, S.K. Mohapatra and V.P. Bondre et al., 2016. Molecular characterization of IFN-T expressed in buffalo embryonic trophoblasts and expression of recombinant BuIFN-T1a2 and BuIFN-T8 isoforms in E. coli. Protein Expression Purif., 122: 8-14.
    CrossRef  |  Direct Link  |  
  10. Rawat, P., S. Bathla, R. Baithalu, M.L. Yadav and S. Kumar et al., 2016. Identification of poteintial protein biomarkers for early detection of pregnancy in cow urine using 2D DiGE and Label free quantitation. Clin. Proteomics, 10.1186/s12014-016-9116-y.
    CrossRef  |  Direct Link  |  
  11. Choudhary, R., S. Kumar, S.V. Singh, A.K. Sharma and T.S. Goud et al., 2016. Validation of putative reference genes for gene expression studies in heat stressed and α-MSH treated melanocyte cells of Bos indicus using real-time quantitative PCR. Mol. Cell. Probes, 30: 161-167.
    CrossRef  |  Direct Link  |  
  12. Anand, V., S. Jaswal, S. Singh, S. Kumar and M.K. Jena et al., 2016. Functional characterization of Mammary Gland Protein-40, a chitinase-like glycoprotein expressed during mammary gland apoptosis. Apoptosis, 21: 209-224.
    CrossRef  |  Direct Link  |  
  13. Saugandhika, S., V. Sharma, H. Malik, S. Saini and S. Bag et al., 2015. Expression and Purification of buffalo interferon-tau and efficacy of recombinant buffalo interferon-tau for in vitro embryo development. Cytokine, 75: 186-196.
    CrossRef  |  Direct Link  |  
  14. Panicker, A.S., P.V. Behare, K. Munjal, S. Kumar and J. Naru et al., 2015. Differential proteome study of putative probiotic Lactobacillus fermentum Bif-19 strain in response to bile stress. J. Proteins Proteomics, 6: 197-210.
  15. Lule, P.V., K. Munjal, S.A. Ali, P. Rawat, S. Kumar, P. Behare and A.K. Mohanty, 2015. Evaluation of stationary phase and bile stress related protein spots in Lactobacillus fermentum NCDC 400 by 2-DE method. Indian J. Dairy Sci., 69: 455-459.
  16. Bathla, S., P. Rawat, R. Baithalu, M.L. Yadav and J. Naru et al., 2015. Profiling of urinary proteins in Karan Fries cows reveals more than 1550 proteins. J. Proteomics, 127: 193-201.
    CrossRef  |  Direct Link  |  
  17. Singhal, D.K., R. Singhal, H.N. Malik, S. Kumar and S. Kumar et al., 2014. Molecular cloning, sequence characterization and recombinant expression of Nanog gene in goat fibroblast cells using lentiviral based expression system. Mol. Biol. Rep., 41: 1907-1915.
    CrossRef  |  Direct Link  |  
  18. Singh, A.K., R.C. Upadhyay, D. Malakar, S. Kumar and S.V. Singh, 2014. Effect of thermal stress on HSP70 expression in dermal fibroblast of zebu (Tharparkar) and crossbred (Karan-Fries) cattle. J. Therm. Biol., 43: 46-53.
    CrossRef  |  Direct Link  |  
  19. Janjanam, J., S. Singh, M.K. Jena, N. Varshney and S. Kola et al., 2014. Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield. PLoS ONE, Vol. 9. 10.1371/journal.pone.0102515.
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  20. Boro, P., A. Kumaresan, A.K. Singh, D. Gupta and S. Kumar et al., 2014. Expression of short chain fatty acid receptors and pro-inflammatory cytokines in utero-placental tissues is altered in cows developing retention of fetal membranes. Placenta, 35: 455-460.
    CrossRef  |  Direct Link  |  
  21. Malik, H.N., D.K. Singhal, S. Saugandhika, A. Dubey and A. Mukherjee et al., 2013. Generation of parthenogenetic goat blastocysts: effects of different activation methods and culture media. Zygote, 23: 327-335.
    CrossRef  |  Direct Link  |  
  22. Malik, H.N., D.K. Singhal, A. Mukherjee, N. Bara and S. Kumar et al., 2013. A single blastomere sexing of caprine embryos by simultaneous amplification of sex chromosome- specific sequence of SRY and amelogenin genes. Livestock Sci., 157: 351-357.
    CrossRef  |  Direct Link  |  
  23. Janjanam, J., M. Jamwal, S. Singh, S. Kumar and A.K. Panigrahi et al., 2013. Proteome analysis of functionally differentiated bovine (Bos indicus) mammary epithelial cells isolated from milk. Proteomics, 13: 3189-3204.
    CrossRef  |  Direct Link  |  
  24. De, A.K., S. Garg, D. Kumar, H.N. Malik and A. Mukherjee et al., 2013. Derivation of goat embryonic stem cell like cell lines from in vitro produced parthenogenetic blastocysts. Small Ruminant Res., 113: 145-153.
    CrossRef  |  Direct Link  |  
  25. Varshney, N., A.K. Mohanty, S. Kumar, J.K. Kaushik and A.K. Dang et al., 2012. Selection of suitable reference genes for quantitative gene expression studies in milk somatic cells of lactating cows (Bos indicus). J. Dairy Sci., 95: 2935-2945.
    CrossRef  |  Direct Link  |  
  26. Janjanam, J., S. Singh, S. Choudhary, M.A. Pradeep and S. Kumar et al., 2012. Molecular cloning, sequence characterization and heterologous expression of buffalo (Bubalus bubalis) oviduct-specific glycoprotein in E. coli. Mol. Biol. Rep., 39: 10031-10043.
    CrossRef  |  Direct Link  |  
  27. Anand, V., N. Dogra, S. Singh, S.N. Kumar and M.K. Jena et al., 2012. Establishment and Characterization of a Buffalo (Bubalus bubalis) Mammary Epithelial Cell Line. PLoS One, 10.1371/journal.pone.0040469.
    CrossRef  |  Direct Link  |  
  28. Pradeep, M.A., J. Jagadeesh, A.K. De, J.K. Kaushik and D. Malakar et al., 2011. Purification, sequence characterization and effect of goat oviduct-specific glycoprotein on in vitro embryo development. Theriogenol., 75: 1005-1015.
    CrossRef  |  Direct Link  |  
  29. Kumar, S., A.Rai, P.K. Gupta and S. Gangwar, 2009. Cloning of Canine Distemper Virus H gene in replicase based eukaryotic vector and analysis of its expression. Biotechnol. Int., 2: 32-45.
  30. Kumar, S., A. Rai, P.K. Gupta and S. Gangwar, 2009. Development of a replicon based DNA vaccine encoding Canine Distemper H gene. Biotechnol. Int., 2: 46-59.
  31. Salunkhe, S.S., A. Rai, A. Saxena, V. Kumar and P.K. Gupta, 2008. Cloning of canine adenovirus type 1 hexon gene in mammalian expression vector and analysis of its immunogenicity. Biotechnol. Int., 1: 22-40.
    Direct Link  |  
  32. Gangwar, S., A. Rai, M. Bagath, S. Kumar and A. Saxena, 2008. Amplification and cloning of human interferon gamma gene in mammalian expression vector and its expression study. Biotechnol. Int., 1: 71-79.