Dr. Muhammad  Irfan

Dr. Muhammad Irfan

Assistant Professor
University of Sargodha, Pakistan


Highest Degree
Ph.D. in Biotechnology from Shenyang Agriculture University, China

Share this Profile

Biography

Dr. Muhammad Irfan is working as an Assistant Professor in Department of Zoology, University of the Punjab new campus Lahore, Pakistan. He received his doctorate degree from Shenyang Agricultural University P.R. China. His research expertise focuses on microbial enzymes from fermentation, lignocellulose biotechnology, single cell protein production and genomics (molecular markers in genetic diversity analysis, QTL mapping in plants). He is also a researcher at Food & Biotechnology Research Center, PCSIR laboratories complex, ferozpure road Lahore Pakistan. He is an editorial/advisory board member of various international repute journals. He has published many research articles in national and international repute journals.

Area of Interest:

Biomedical Sciences
100%
Protease
62%
Amylase
90%
Cellulase
75%
Biotechnology
55%

Selected Publications

  1. Tabssum, F., M. Irfan, H.A. Shakir and J.I. Qazi, 2017. Statistical optimization for deconstruction of poplar substrate by dilute sulphuric acid for bioethanol production. Green Chem. Lett. Rev., 10: 69-79.
    CrossRef  |  Direct Link  |  

  2. Siddique, A., A. Gul, M. Irfan, M. Nadeem and M. Syed, 2017. Comparison of different pretreatment methods for efficient conversion of bagasse into ethanol. Biofuels, 8: 135-141.
    CrossRef  |  Direct Link  |  

  3. Irfan, M., S. Yang, L. Yuxin and J. Sun, 2017. Genetic diversity analysis of Morchella sp. by RAPD. Mol. Biol. Res. Commun., 6: 27-31.
    Direct Link  |  

  4. Irfan, M., Q. Mushtaq, F. Tabassum, H.A. Shakir and J.I. Qazi, 2017. Carboxymethyl Cellulase production optimization from newly isolated thermophilic Bacillus subtilis K-18 for saccharification using response surface methodology. AMB Exp., Vol. 7. 10.1186/s13568-017-0331-3.
    CrossRef  |  

  5. Chen, L., Z. Li, W. Qi, M. Irfan and J. Lin et al., 2017. Characterization of promoter region of glycerol-3-phosphate-O-acyltransferase gene in Lilium pensylvanicum. Turk. J. Biol., Vol. 41. .
    Direct Link  |  

  6. Arooj, A., M. Irfan, F. Tabsum, H.A. Shakir and J.I. Qazi, 2017. Effect of dilute sulphuric acid pretreatment on cellulase production by Bacillus subtilis K-18 through response surface methodology. Proc. Pak. Acad. Sci. B: Life Environ. Sci., 54: 11-20.
    Direct Link  |  

  7. Ahsan, T., J. Chen, Y. Wu, M. Irfan and J. Shafi, 2017. Screening, identification, optimization of fermentation conditions and extraction of secondary metabolites for the biocontrol of Rhizoctonia solani AG-3. Biotechnol. Biotechnol. Equipment, 31: 91-98.
    CrossRef  |  Direct Link  |  

  8. Ahsan, T., J. Chen, Y. Wu and M. Irfan, 2017. Application of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460. AMB Exp. 10.1186/s13568-017-0388-z.
    CrossRef  |  

  9. Ahsan, T., J. Chen, X. Zhao, M. Irfan and Y. Wu, 2017. Extraction and identification of bioactive compounds (Eicosane and Dibutyl phthalate) produced by Streptomyces strain KX852460 for the Biological control of Rhizoctonia solani AG-3 strain KX852461 to control Target spot disease in tobacco leaf. AMB Exp., Vol. 7 10.1186/s13568-017-0351-z.
    CrossRef  |  

  10. Wang, N., W. Hong, H.Y. Liu, M. Irfan, S.W. Niu and Y. Tao, 2016. Diversity of methane-producing microbial community structure with 16S-V3 rDNA PCR-DGGE. Genetika, 48: 1111-1119.
    Direct Link  |  

  11. Siddique, S., H.A. Shakir, J.I. Qazi, A.B. Tabinda and M. Irfan, 2016. Screening of some agri-wastes for economical cultivation of Candida tropicalis SS1. Punjab Univ. J. Zool., 31: 31-37.
    Direct Link  |  

  12. Shakir, H.A., J.I. Qazi, M. Irfan and S. Ali, 2016. Alteration in biochemical response of Indian major carp Labeo rohita exposed to urban effluents discharged in the river Ravi Pakistan. Biologia, 62: 219-227.
    Direct Link  |  

  13. Shahid, Z.H., M. Irfan, M. Nadeem, Q. Syed and J.I. Qazi, 2016. Production, purification, and characterization of carboxymethyl cellulase from novel strain Bacillus megaterium. Environ. Prog. Sustainable Energy, 35: 1741-1749.
    CrossRef  |  Direct Link  |  

  14. Mushtaq, Q., M. Irfan, F. Tabssum and J.I. Qazi, 2016. Potato peels: A potential food waste for amylase production. J. Food Process Eng., 10.1111/jfpe.12512.
    CrossRef  |  Direct Link  |  

  15. Majeed, H.S., M. Irfan, H.A. Shakir and J.I. Qazi, 2016. Filter paper activity producing potential of Aeromonas species isolated from the gut of Labeo rohita. Pak. J. Zool., 48: 1317-1323.
    Direct Link  |  

  16. Mahmood, S., M.G. Shahid, M. Nadeem, M. Irfan and Q. Syed, 2016. Production and optimization of α-amylase from Aspergillus niger using potato peel as substrate. Pak. J. Biotechnol., 13: 101-109.

  17. Karim, A., W. Ali, N. Ahmad, M. Irfan and H.A. Shakir, 2016. Histological and Biochemical study of liver of silver carp (Hypophthalmichthys molitrix) after acute exposure to pyrethoride (deltamethrin). Punjab Univ. J. Zool., 31: 229-236.
    Direct Link  |  

  18. Jia B., X. Zhao, Y. Qin, M. Irfan and T.H. Kim, 2016. Identification of quantitative trait loci for leaf traits in rice. Genetika, 48: 643-652.

  19. Irfan, M., U. Asghar, M. Nadeem, R. Nelofer, Q. Syed, H.A. Shakir and J.I. Qazi, 2016. Statistical optimization of saccharification of alkali pretreated wheat straw for bioethanol production. Waste Biomass Valorization, 7: 1389-1396.
    CrossRef  |  Direct Link  |  

  20. Irfan, M., U. Asghar, M. Nadeem, R. Nelofer and Q. Syed, 2016. Optimization of process parameters for xylanase production by Bacillus sp. in submerged fermentation. J. Radiat. Res. Appl. Sci., 9: 139-147.
    CrossRef  |  Direct Link  |  

  21. Irfan, M., U. Asghar, M. Nadeem, H.A. Shakir, J.I. Qazi, R. Nelofer and Q. Syed, 2016. Process optimization for endoglucanase production by Trichoderma harzianum in submerged fermentation. Hacettepe J. Biol. Chem., 44: 87-93.

  22. Irfan, M., M. Nadeem, Q. Syed, H.A. Shakir and J.I. Qazi, 2016. Study on Some Properties of Calcium-dependent a-Amylase from Bacillus subtilis through submerged fermentation of wheat bran. Chem. Biochem. Eng. Q., 30: 429-437.
    CrossRef  |  

  23. Irfan, M., M. Nadeem, Q. Syed and J.I. Qazi, 2016. Statistical optimization of dilute acid pretreatment of pinus needles to be used as substrate for biofuel production. Energy Sources Part A Recovery Util. Environ. Effects, 38: 1983-1992.
    CrossRef  |  Direct Link  |  

  24. Deeba, F., H.A. Shakir, M. Irfan and J.I. Qazi, 2016. Chitinase production in organisms: A review. Punjab Univ. J. Zool., 31: 101-106.
    Direct Link  |  

  25. Ashgar, U., M. Nadeem, M. Irfan, Q. Syed, R. Nelofer and M. Iram, 2016. Effect of NaOH on delignification of Saccharum spontaneum. Environ. Prog. Sustainable Energy, 35: 284-288.
    CrossRef  |  Direct Link  |  

  26. Asghar, U., M. Irfan, M. Nadeem and Q. Syed, 2016. Effect of NaOH on pretreatment of gossypium herbaceum. Energy Sources Part A Recovery Util. Environ. Effects, 38: 1898-1903.
    CrossRef  |  Direct Link  |  

  27. Wang, Y., W. Liu, Y. Jiang, L. Huang and M. Irfan et al., 2015. Morphological and molecular characterization of Nosema pernyi, a microsporidian parasite in Antheraea pernyi. Parasitol. Res., 114: 3327-3336.
    CrossRef  |  PubMed  |  Direct Link  |  

  28. Wang, H., X. Zhao, C. Liu, L. Liu and S. Yu et al., 2015. Effects of the biocontrol agent Aspergillus flavipes on the soil microflora and soil enzymes in the rooting zone of pepper plants infected with Phytophthora capsici. J. Phytopathol., 163: 513-521.
    CrossRef  |  Direct Link  |  

  29. Sun, S., N. Yang, L. Chen, M. Irfan, X. Zhao and T. Li, 2015. Characterization of LpGPAT gene in Lilium pensylvanicum and response to cold stress. Biomed. Res. Int., Vol. 2015. 10.1155/2015/792819.
    CrossRef  |  Direct Link  |  

  30. Nadeem, M., M.U. Aftab, M. Irfan, M. Mushtaq, A. Qadir and Q. Syed, 2015. Production of ethanol from alkali-pretreated sugarcane bagasse under the influence of different process parameters. Front. Life Sci., 8: 358-362.
    CrossRef  |  Direct Link  |  

  31. Mushtaq, Z., M. Irfan, M. Nadeem, M. Naz and Q. Syed, 2015. Kinetics study of extracellular detergent stable alkaline protease from rhizopus oryzae. Braz. Arch. Biol. Technol., 58: 175-184.
    CrossRef  |  Direct Link  |  

  32. Li, J., Y. Yi, X. Cheng, D. Zhang and M. Irfan, 2015. Study on the effect of magnetic field treatment of newly isolated Paenibacillus sp. Botanical Stud., Vol. 56. 10.1186/s40529-015-0083-9.
    CrossRef  |  

  33. Iqbal, M.N., A.A. Anjum, M.A. Ali, F. Hussain and S. Ali et al., 2015. Assessment of microbial load of un-pasteurized fruit juices and in vitro antibacterial potential of honey against bacterial isolates. Open Microbiol. J., 9: 26-32.

  34. Chaudhary, N., J.I. Qazi and M. Irfan, 2015. Isolation and identification of cellulolytic and ethanologenic bacteria from soil. Iran. J. Sci. Technol., .
    Direct Link  |  

  35. Asghar, U., M. Irfan, M. Nadeem, R. Nelofer and Q. Syed, 2015. Effect of KOH pretreatment on lignocellulosic waste to be used as substrate for ethanol production. Iran. J. Sci. Technol., .
    Direct Link  |  

  36. Asghar, U., M. Irfan, M. Nadeem, R. Nelofer and Q. Syed, 2015. Challenges in bioethanol production from lignocellulosic waste. Energy Educ. Sci. Technol. Part C Future Energy Resour., 7: 7-16.

  37. Asghar, U., M. Irfan, M. Iram, Z. Huma, R. Nelofer, M. Nadeem and Q. Syed, 2015. Effect of alkaline pretreatment on delignification of wheat straw. Nat. Prod. Res., 29: 125-131.
    CrossRef  |  Direct Link  |  

  38. Zhou, T., M. Fan, M. Irfan, H. Wang and D. Wang et al., 2014. Phylogenetic analysis of STK gene family and Usp domain in maize. Mol. Biol. Rep., 41: 8273-8284.
    CrossRef  |  

  39. Wang, D., H. Wang, M. Irfan, M. Fan and F. Lin, 2014. Structure and evolution analysis of pollen receptor-like kinase in Zea mays and Arabidopsis thaliana. Comput. Biol. Chem., 51: 63-70.
    CrossRef  |  Direct Link  |  

  40. Li, C., M. Irfan, C. Zhang and F. Lin, 2014. Genetic diversity analysis of maize varities based on SSR markers. Res. J. Biotechnol., 9: 48-51.

  41. Khokhar, Z., Q. Syed, M. Nadeem, M. Irfan and J. Wu et al., 2014. Enhanced production of cellulase by Trichoderma reesei using wheat straw as a carbon source. World Applied Sci. J., 30: 1095-1104.
    Direct Link  |  

  42. Irfan, M., M. Nadeem and Q. Syed, 2014. One-factor-at-a-time (OFAT) optimization of xylanase production from Trichoderma viride-IR05 in solid-state fermentation. J. Radiat. Res. Applied Sci., 7: 317-326.
    CrossRef  |  Direct Link  |  

  43. Irfan, M., M. Nadeem and Q. Syed, 2014. Ethanol production from agricultural wastes using Saccharomyces cervisae. Braz. J. Microbiol., 42: 457-465.

  44. Irfan, M., J.X. Sun, Y. Liu, X. Li and S. Yang, 2014. Genetic analysis of chlorophyll content in maize by mixed major and polygene models. Genetika, 46: 1037-1046.
    Direct Link  |  

  45. Hu, M., X. Zhou, Y. Shi, J. Lin, M. Irfan and Y. Tao, 2014. Essential role of the N-and C-terminals of laccase from Pleurotus florida on the laccase activity and stability. Applied Biochem. Biotechnol., 174: 2007-2017.
    CrossRef  |  Direct Link  |  

  46. Yang, S., M. Irfan, X. Li, Y. Liu and X. Sun, 2013. Detection of Bt gene and seed purity in maize. Hacettepe J. Biol. Chem., 41: 341-346.

  47. Wang, Y., S. Yang, M. Irfan, C. Zhang, Q. Sun, S. Wu and F. Lin, 2013. Genetic analysis of carbon metabolism-related traits in maize using mixed major and polygene models. Aust. J. Crop Sci., 7: 1205-1211.
    Direct Link  |  

  48. Siddique, S., Q. Syed, A. Adnan, M. Nadeem, M. Irfan and F.A. Qureshi, 2013. Production of avermectin B1b from Streptomyces avermitilis 41445 by batch submerged fermentation. Jundishapur J. Microbiol., Vol. 6. 10.5812/jjm.7198.
    CrossRef  |  Direct Link  |  

  49. Irfan, M., Z.T. Ting, W. Yang, Z. Chunyu and M. Qing et al., 2013. Modification of CTAB protocol for maize genomic DNA extraction. Res. J. Biotechnol., 8: 41-45.
    Direct Link  |  

  50. Irfan, M., M. Nadeem and Q. Syed, 2013. Purification and characterization of xylanase from Bacillus subtilis. Protein Peptide Lett., 20: 1225-1231.

  51. Irfan, M., M. Nadeem and Q. Syed, 2013. Production of cellulases and hemicellulases from cellulytic fungal cultures in submerged fermentation using agricultural wastes. Electron. J. Biol., 9: 62-66.

  52. Bai, H., H. Wang, J. Sun, M. Irfan and M. Han et al., 2013. Purification and characterization of beta 1, 4-Glucanases from Penicillium simplicissimum H-11. BioResources, 8: 3657-3671.
    Direct Link  |  

  53. Bai, H., H. Wang, J. Sun, M. Irfan and M. Han et al., 2013. Production, purification and characterization of novel beta glucosidase from newly isolated Penicillium simplicissimum H-11 in submerged fermentation. EXCLI J., 12 528.
    CrossRef  |  ASCI  |  Direct Link  |  

  54. Su, D., J. Fu and M. Irfan, 2012. First Record of Didymella anemones sp. nov. Teleomorph of Ascochyta anemones, the Causal Agent of Leaf Spots on Pulsatilla korreana in China. J. Phytopathol., 160: 728-731.
    CrossRef  |  Direct Link  |  

  55. Sher, M.G., M. Nadeem, Q. Syed, M. Irfan and S. Baig, 2012. Protease production from UV mutated Bacillus subtilis. Bangladesh J. Sci. Ind. Res., 47: 69-76.
    Direct Link  |  

  56. Irfan, M., M. Nadeem, Q. Syed and S. Baig, 2012. Effect of medium composition on xylanase production by Bacillus subtilis using various agricultural wastes. American-Eurasian J. Agric. Environ. Sci., 12: 561-565.
    Direct Link  |  

  57. Irfan, M., M. Nadeem and Q. Syed, 2012. Media optimization for amylase production in solid state fermentation of wheat bran by fungal strains. J. Cell. Mol. Biol., 10: 55-64.

  58. Irfan, M., M. Nadeem and Q. Syed, 2012. Influence of nutritional conditions for endoglucanase production by Trichoderma viride in SSF. Global J. Biotechnol. Biochem., 7: 7-12.
    CrossRef  |  Direct Link  |  

  59. Irfan, M., A. Safdar, Q. Syed and M. Nadeem, 2012. Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity. Turk. J. Biochem., 37: 287-293.
    CrossRef  |  Direct Link  |  

  60. Irfan, M. and Q. Syed, 2012. Partial purification and characterization of xylanase from Trichoderma viride produced under SSF. Int. J. Appl. Res. Nat. Prod., 5: 7-11.
    Direct Link  |  

  61. Irfan, M., U. Irfan, Z. Razzaq, Q. Syed and M. Nadeem, 2011. Utilization of agricultural wastes as a substrate for Carboxymethyl cellulase production from Aspergillus niger in submerged fermentation. IJAVMS, 5: 464-471.
    CrossRef  |  Direct Link  |  

  62. Irfan, M., Q. Syed, S. Abbas, M. GulSher, S. Baig and M. Nadeem, 2011. FTIR and SEM analysis of thermo-chemical fractionated of Sugarcane Bagasse. Turkish J. Biochem., 36: 322-328.
    Direct Link  |  

  63. Irfan, M., M.I. Nazir, M. Nadeem, M. Gulsher, Q.A. Syed and S. Baig, 2011. Optimization of process parameters for the production of single cell biomass of Candida utilis in solid state fermentation. Am-Eurasian J. Agric. Environ. Sci., 10: 264-270.
    Direct Link  |  

  64. Irfan, M., M. Nadeem, Q. Syed and S. Baig, 2011. Production of thermostable α-amylase from Bacillus sp. in solid state fermentation. J. Appl. Sci. Res., 7: 607-617.

  65. Irfan, M., M. Gulsher, S. Abbas, Q. Syed, M. Nadeem and S. Baig, 2011. Effect of various pretreatment conditions on enzymatic saccharification. Songklanakarin J. Sci. Technol., 33: 397-404.
    Direct Link  |  

  66. Irfan, M., J. Javed and Q. Syed, 2011. UV mutagenesis of Aspergillus niger for enzyme production in submerged fermentation. Pak. J. Biochem. Mol. Biol., 44: 137-140.
    Direct Link  |  

  67. Irfan, M., A., Rauf, Q.A. Syed, M. Nadeem and S. Baig, 2011. Exploitation of different agro-residues for acid protease production by Rhizopus sp. in Koji fermentation. Int. J. Agr. Vet. Med. Sci., 5: 43-52.
    CrossRef  |  Direct Link  |  

  68. Irfan, M., 2011. Trichoderma sp. A potent producer of xylanase enzyme: Xylanase production in solid state fermentation. Lap Lambert Academic Publishing GmbH and Co. KG, Germany., ISBN: 978-3847316176, Pages: 72..
    Direct Link  |  

  69. Irfan, M. and Q. Syed, 2011. Effect of 2-mercaptoethanol on enzymes secretion by Aspergillus niger in submerged fermentation. Pak. J. Biochem. Mol. Biol., 44: 160-163.
    Direct Link  |  

  70. Iqbal, H.M.N., I. Ahmed, M.A. Zia and M. Irfan, 2011. Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility. Adv. Biosci. Biotechnol., 2: 149-156.
    CrossRef  |  Direct Link  |  

  71. Yousaf, M., M. Irfan, Z.U. Khokhar, Q.A. Syed, S. Baig and A. Iqbal, 2010. Enhanced production of Protease by mutagenized strain of Aspergillus oryzae in solid substrate fermentation of rice bran. Sci. Int. (Lahore)., 22: 119-123.

  72. Rauf, A., M. Irfan, M. Nadeem, I. Ahmed and H.M.N. Iqbal, 2010. Optimization of growth conditions for acidic protease production from Rhizopus oligosporus through solid state fermentation of sunflower meal. World Acad. Sci. Eng. Technol., 72: 608-611.
    Direct Link  |  

  73. Nadeem, A., Q. Syed, S. Baig, M. Irfan and M. Nadeem, 2010. Enhanced production of citric acid by Aspergillus niger M-101 using lower alcohols. Turk. J. Biochem., 35: 7-13.
    Direct Link  |  

  74. Munawar, R.A., M. Irfan, M. Nadeem, Q.A. Syed and Z.H. Siddique, 2010. Biosynthesis of single cell biomass of Candida utilis by submerged fermentation. Pak. J. Sci., 62: 1-5.
    Direct Link  |  

  75. Khokhar, Z., Q.A. Syed, S. Baig, M. Nadeem, M. Irfan and M.A. Athar, 2010. Study on filtrate obtained from batch-wise pretreatment of wheat straw at different concentrations of h2so4 and autoclave time. World Applied Sci. J., 10: 1047-1055.
    Direct Link  |  

  76. Khokhar, Z., Q. Syed, M. Nadeem, S. Baig and M. Irfan et al., 2010. Delignification of wheat straw with acid and hydro-steam under pressure. World Appl. Sci. J., 11: 1524-1530.

  77. Irfan, M., S. Abbas, S. Baig, M. Gulsher, M. Nadeem and Q.A. Syed, 2010. Pretreatment: A potential technique to enhance the Enzymatic hydrolysis. World J. Agric. Sci., 6: 440-445.

  78. Irfan, M., Q.A. Syed, M. Gulsher, S. Abbas, M. Nadeem and S. Baig, 2010. Pretreatment of corncobs for the production of hydrolytic enzymes from Aspergillus niger-IR01. Int. J Agric. Vet. Med. Sci., 4: 81-87.
    Direct Link  |  

  79. Irfan, M., Q. Syed, M. Yousaf, M. Nadeem, S. Baig and S.A. Jafri, 2010. Studies on the pretreatment of wheat straw for improve production of carboxymethyl cellulase by Trichoderma viride- FBL1 in solid state fermentation. Acad. Arena., 2: 18-30.

  80. Irfan, M., M. Nadeem, Q.A. Syed and S. Baig, 2010. Submerged cultivation of Aspergillus niger on pretreated sugarcane bagasse. World J. Agric. Sci., 6: 466-472.
    Direct Link  |  

  81. Irfan, M., M. Nadeem, Q. Syed, W. Nawaz, S. Baig and A.M. Qureshi, 2010. Relationship of process parameters for the production of alkaline protease by Bacillus sp. Int. J. Agr. Vet. Med. Sci., 4: 114-120.
    Direct Link  |  

  82. Hayat, M.U., M. Irfan, A.A.N. Saqib, M. Nadeem, Q.A. Syed and S. Baig, 2010. Degradation of cellulose by co-cultures and the mixture of culture filtrates. Sci. Int. (Lahore)., 22: 303-306.

  83. Gulsher, M., S. Abbas, M. Irfan, M. Nadeem, Q.A. Syed and S. Baig, 2010. Biosynthesis of cellulase from bagasse with fungal strains. Pak. J. Biochem. Mol. Biol., 43: 146-148.

  84. Gulsher, M., M. Nadeem, Q.A. Syed, M. Irfan and S. Baig, 2010. Alkaline protease production from industrial waste by Bacillus subtilis ML-4. Pak. J. Sci. Ind. Res., 53: 146-150.

  85. Ahmad, I., M. Irfan, M. Nadeem, M.A. Zia, B.M. Ahmad and H.M.N. Iqbal, 2010. Relationship of process parameters for the production of alkaline protease by Bacillus sp. Int. J. Agr. Vet. Med. Sci., 4: 105-113.

  86. Syed, Q.A., M. Irfan, M. Nadeem and S. Baig, 2009. Production of neutral proteases from rice bran using Aspergillus oryzae in solid state fermentation. Pak. J. Biochem. Mol. Biol., 42: 147-152.

  87. Nadeem, A., S.A. Jafri, S. Baig, M. Irfan and Q.A. Syed, 2009. Optimization of substrate concentration for enhanced citric acid production using Aspergillus niger M-101. Pak. J. Sci. Ind. Res., 52: 275-277.

  88. Irfan, M., M. Gulsher, M. Nadeem, Q.A. Syed and S. Baig, 2009. Submerged production of alkaline protease by newly isolated Bacillus sp. Pak. J. Food Sci., 19: 12-16.
    Direct Link  |  

  89. Irfan, M., M. Gulsher, M. Nadeem and Q.A. Syed, 2009. Evaluation of cultural conditions for thermostable α-amylase production from Bacillus sp. production. Pak. J. Biochem. Mol. Biol., 42: 43-48.
    Direct Link  |  

  90. Irfan, M., M. Nadeem, S. Baig, Q.A. Syed and A.M. Qureshi, 2008. Purification and characterization of protease from Bacillus spp. Pak. J. Biochem. Mol. Biol., 41: 129-132.